Literature summary for 3.4.22.B79 extracted from

Gorchakov, R.; Frolova, E.; Sawicki, S.; Atasheva, S.; Sawicki, D.; Frolov, I.
A new role for ns polyprotein cleavage in Sindbis virus replication (2008), J. Virol., 82, 6218-6231.

Search for more literature for 3.4.22.B79

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in BHK-21 cells	Sindbis virus

Protein Variants

Protein Variants	Comment	Organism
additional information	ns polyprotein cleavage mutants of Sindbis virus differ from the Sindbis virus/GFP variant in their ability to inhibit cellular translation and transcription. The nsP2/nsP3 cleavage site mutant replicates in BHK-21 cells almost as efficiently as the cleavage competent Sindbis virus/GFP. In the case of the 1V2V ns polyprotein mutant, only one additional substitution in the nsP4 protein sequence is required to strongly increase RNA replication (but not the transcription of the subgenomic RNA) to the wild-type level. Ns polyprotein cleavage mutants induce a strong interferon-alpha/beta response	Sindbis virus

Organism

Organism	UniProt	Comment	Textmining
Sindbis virus	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	after negative-strand synthesis, the ns protein appears to irreversibly change conformation and forms mature replication complexes, in spite of the lack of ns polyprotein cleavage. Complete processing of the ns polyprotein is not an absolute prerequisite for Sindbis virus replication. Inability of the viruses with unprocessed polyprotein to productively replicate in the interferon-competent cells and in the cells of mosquito origin is an additional, important factor in ns polyprotein cleavage development. It leads to the release of nsP2 protein, which plays a critical role in inhibiting the cellular antiviral response	Sindbis virus	?	-	?

Synonyms

Synonyms	Comment	Organism
nonstructural polyprotein	-	Sindbis virus
ns polyprotein	-	Sindbis virus

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Release 2023.1 (January 2023)