Literature summary for 3.4.22.B79 extracted from

Atasheva, S.; Gorchakov, R.; English, R.; Frolov, I.; Frolova, E.
Development of Sindbis viruses encoding nsP2/GFP chimeric proteins and their application for studying nsP2 functioning (2007), J. Virol., 81, 5046-5057.

Search for more literature for 3.4.22.B79

Application

Application	Comment	Organism
additional information	nsP2/GFP is capable of efficient functioning in Sindbis virus replication complexes that can synthesize RNA and, ultimately, produce virus at a level comparable to that of wild-type Sindbis virus. GFP inserted into nsP2 is accessible to specific antibodies and capable of functioning as an efficient tag for the isolation of protein complexes formed during Sindbis virus replication	Sindbis virus

Cloned(Commentary)

Cloned (Comment)	Organism
GFP-encoding DNA fragment cloned into the nsP2 gene by using a transposon-based approach. The entire library of the recombinant nsP2/GFP genes transferred into wild-type Sindbis virus Toto1101 genome. SIN/nsP2GFP/8 and SIN/nsP2GFP/472 expressed in BHK-21 cells	Sindbis virus

Protein Variants

Protein Variants	Comment	Organism
additional information	SIN/2V/389 virus, has GFP insertion after aa 389 of nsP3 and a single-point mutation leading to replacement of glycine by valine in the P2 position of the cleavage site between nsP2 and nsP3. Blocks polyprotein processing	Sindbis virus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytoplasm	SIN/nsP2GFP/8-specific fusion protein is present in the cytoplasm in large complexes. SIN/nsP2GFP/472 also present	Sindbis virus	5737	-
nucleus	very little amounts of SIN/nsP2GFP/8-specific fusion protein. SIN/nsP2GFP/472 also present	Sindbis virus	5634	-

Organism

Organism	UniProt	Comment	Textmining
Sindbis virus	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Sindbis virus core protein + H2O	-	Sindbis virus	Hydrolyzed Sindbis core protein	-	?

Synonyms

Synonyms	Comment	Organism
nsP2	-	Sindbis virus

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Release 2023.1 (January 2023)