BRENDA - Enzyme Database show
show all sequences of 3.4.22.B75

Differential expression of SUMO-specific protease 7 variants regulates epithelial-mesenchymal transition

Bawa-Khalfe, T.; Lu, L.S.; Zuo, Y.; Huang, C.; Dere, R.; Lin, F.M.; Yeh, E.T.; Proc. Natl. Acad. Sci. USA 109, 17466-17471 (2012)

Data extracted from this reference:

Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
chromatin
-
Homo sapiens
785
-
nucleus
-
Homo sapiens
5634
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
SUMO2/3ylated HP1alpha + H2O
Homo sapiens
HP1alpha localizes at the pericentric heterochromatin, importance of SUMOylation in directing HP1alpha’s subnuclear localization, SUMO deconjugation by enzyme SENP7
HP1alpha + SUMO3 + SUMO2
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
two splicing variants, a short SENP7 splice variant SENP7S and a long transcript SENP7L
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
breast cancer cell
-
Homo sapiens
-
breast epithelial cell
two splicing variants, a short SENP7 splice variant SENP7S and a long transcript SENP7L
Homo sapiens
-
MDA-MB-231 cell
-
Homo sapiens
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
long enzyme variant SENP7L, but not short enzyme variant SENP7S, binds HP1alpha. This interaction does not require catalytic activity: both the active wild-type SENP7L and the catalytically inactive mutant (SENP7Lm) bind endogenous HP1alpha efficiently
732812
Homo sapiens
?
-
-
-
-
SUMO2/3ylated HP1alpha + H2O
HP1alpha localizes at the pericentric heterochromatin, importance of SUMOylation in directing HP1alpha’s subnuclear localization, SUMO deconjugation by enzyme SENP7
732812
Homo sapiens
HP1alpha + SUMO3 + SUMO2
-
-
-
?
SUMO2/3ylated HP1alpha + H2O
SUMO deconjugation by enzyme SENP7
732812
Homo sapiens
HP1alpha + SUMO3 + SUMO2
-
-
-
?
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
chromatin
-
Homo sapiens
785
-
nucleus
-
Homo sapiens
5634
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
SUMO2/3ylated HP1alpha + H2O
Homo sapiens
HP1alpha localizes at the pericentric heterochromatin, importance of SUMOylation in directing HP1alpha’s subnuclear localization, SUMO deconjugation by enzyme SENP7
HP1alpha + SUMO3 + SUMO2
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
breast cancer cell
-
Homo sapiens
-
breast epithelial cell
two splicing variants, a short SENP7 splice variant SENP7S and a long transcript SENP7L
Homo sapiens
-
MDA-MB-231 cell
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
long enzyme variant SENP7L, but not short enzyme variant SENP7S, binds HP1alpha. This interaction does not require catalytic activity: both the active wild-type SENP7L and the catalytically inactive mutant (SENP7Lm) bind endogenous HP1alpha efficiently
732812
Homo sapiens
?
-
-
-
-
SUMO2/3ylated HP1alpha + H2O
HP1alpha localizes at the pericentric heterochromatin, importance of SUMOylation in directing HP1alpha’s subnuclear localization, SUMO deconjugation by enzyme SENP7
732812
Homo sapiens
HP1alpha + SUMO3 + SUMO2
-
-
-
?
SUMO2/3ylated HP1alpha + H2O
SUMO deconjugation by enzyme SENP7
732812
Homo sapiens
HP1alpha + SUMO3 + SUMO2
-
-
-
?
General Information
General Information
Commentary
Organism
malfunction
loss of SENP7LHP1alpha interaction causes HP1alpha hyperSUMOylation, an enrichment of HP1alpha at E2F-responsive and mesenchymal gene promoters, silences transcription of these genes, and elicits cellular senescence. Reducing all catalytically active SENP7 isoforms prompts a reduction in GILM2 cells' invasiveness. Lower mRNA of E2F-responsive genes and vimentin in tumor mass from GI101a-shSENP7 and GILM2-shSENP7 than their respective shNT control xenografts
Homo sapiens
physiological function
induction of SENP7L long variant maintains hypoSUMOylated HP1alpha, which relieves HP1alpha-mediated repression of proliferation promoting E2F-responsive genes as well as mesenchymal genes
Homo sapiens
General Information (protein specific)
General Information
Commentary
Organism
malfunction
loss of SENP7LHP1alpha interaction causes HP1alpha hyperSUMOylation, an enrichment of HP1alpha at E2F-responsive and mesenchymal gene promoters, silences transcription of these genes, and elicits cellular senescence. Reducing all catalytically active SENP7 isoforms prompts a reduction in GILM2 cells' invasiveness. Lower mRNA of E2F-responsive genes and vimentin in tumor mass from GI101a-shSENP7 and GILM2-shSENP7 than their respective shNT control xenografts
Homo sapiens
physiological function
induction of SENP7L long variant maintains hypoSUMOylated HP1alpha, which relieves HP1alpha-mediated repression of proliferation promoting E2F-responsive genes as well as mesenchymal genes
Homo sapiens
Other publictions for EC 3.4.22.B75
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
731599
Gonzalez-Prieto
c-Myc is targeted to the prote ...
Homo sapiens
Cell Cycle
14
1859-1872
2015
-
-
-
-
1
-
-
-
1
-
-
1
-
1
-
-
-
-
-
5
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
5
-
-
2
-
-
-
-
-
-
-
-
-
-
4
4
-
-
-
731614
Romeo
The SENP7 SUMO-protease presen ...
Mus musculus
Cell Rep.
10
771-782
2015
-
-
1
-
1
-
-
-
1
-
-
2
-
2
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
3
3
-
-
-
732884
Alegre
Structural insights into the S ...
Homo sapiens
Protein Sci.
23
433-441
2014
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
731715
Garvin
The deSUMOylase SENP7 promotes ...
Homo sapiens
EMBO Rep.
14
975-983
2013
-
-
1
-
2
-
-
-
1
-
-
2
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
3
3
-
-
-
732566
Maison
The SUMO protease SENP7 is a c ...
Mus musculus
Nat. Struct. Mol. Biol.
19
458-460
2012
-
-
1
-
2
-
-
-
1
-
-
1
-
2
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
732812
Bawa-Khalfe
Differential expression of SUM ...
Homo sapiens
Proc. Natl. Acad. Sci. USA
109
17466-17471
2012
-
-
-
-
-
-
-
-
2
-
-
1
-
1
-
-
-
-
-
3
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
3
-
-
3
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
714083
Shen
Characterization of SENP7, a S ...
Homo sapiens
Biochem. J.
421
223-230
2009
-
-
1
-
-
-
-
-
1
-
-
1
-
1
-
-
1
-
-
-
-
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
1
-
-
-
-
4
-
1
-
-
-
1
-
-
-
-
2
2
-
-
-
714081
Drag
Activity profiling of human de ...
Homo sapiens
Biochem. J.
409
461-469
2008
2
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
3
-
1
-
-
-
1
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
715494
Lima
Structure of the human SENP7 c ...
Homo sapiens
J. Biol. Chem.
283
32045-32055
2008
-
-
-
1
4
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
8
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
4
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
8
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-