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show all sequences of 3.4.22.B75

Structure of the human SENP7 catalytic domain and poly-SUMO deconjugation activities for SENP6 and SENP7

Lima, C.D.; Reverter, D.; J. Biol. Chem. 283, 32045-32055 (2008)

Data extracted from this reference:

Crystallization (Commentary)
Crystallization
Organism
crystals of the SENP7 catalytic domain are obtained at 18°C by sitting drop vapor diffusion methods crystal structure of the SENP7 catalytic domain at a resolution of 2.4 A
Homo sapiens
Engineering
Amino acid exchange
Commentary
Organism
C745K
mutation does not alter deconjugation rates in comparison with wild-type SENP7
Homo sapiens
F709W
end point deconjugation reactions using di-SUMO2/3 or poly-SUMO2/3 reveales rates equivalent to or slightly greater than wild-type SENP7. Mutation results in a 2fold higher activity when di-SUMO2 deconjugation rates are measured
Homo sapiens
V713E
mutation elicits 65-fold reduction in deconjugation rate compared to wild-type activity
Homo sapiens
V713E/C745K
C745K substitution partially rescues defects observed for SENP7-V713E when present as a double point substitution (SENP7-V713E/C745K)
Homo sapiens
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
Q9BQF6
-
-
Purification (Commentary)
Commentary
Organism
-
Homo sapiens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(SUMO-3)-Ran GTPase-activating protein 1 conjugate + H2O
SENP6 and SENP7 prefer SUMO2 or SUMO3 in deconjugation reactions with rates comparable with those catalyzed by SENP2. In contrast to SENP2, SENP6 and SENP7 are less able to deconjugate SUMO1-RanGAP1, and products are only detected at enzyme concentrations 100 x higher than that observed for reactions containing SENP2
715494
Homo sapiens
SUMO-3 + Ran GTPase-activating protein 1
-
-
-
?
di-SUMO-2 + H2O
-
715494
Homo sapiens
2 SUMO-2
-
-
-
?
di-SUMO-3 + H2O
-
715494
Homo sapiens
2 SUMO-3
-
-
-
?
poly-SUMO-2 + H2O
-
715494
Homo sapiens
?
-
-
-
?
poly-SUMO-3 + H2O
-
715494
Homo sapiens
?
-
-
-
?
SUMO-1 precursor + H2O
SENP7 exhibits lower rates for processing pre-SUMO-1, pre-SUMO-2, or pre-SUMO-3 in comparison with SENP2
715494
Homo sapiens
SUMO-1 + His-Ser-Thr-Val
-
-
-
?
SUMO-2 precursor + H2O
SENP7 exhibits lower rates for processing pre-SUMO1, pre-SUMO-2, or pre-SUMO-3 in comparison with SENP2
715494
Homo sapiens
SUMO-2 + Val-Tyr
-
-
-
?
SUMO-3 precursor + H2O
SENP7 exhibits lower rates for processing pre-SUMO1, pre-SUMO-2, or pre-SUMO-3 in comparison with SENP2
715494
Homo sapiens
SUMO-3 + Val-Pro-Glu-Ser-Ser-Leu-Ala-Gly-His-Ser-Phe
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
23
-
assay at
Homo sapiens
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Homo sapiens
Crystallization (Commentary) (protein specific)
Crystallization
Organism
crystals of the SENP7 catalytic domain are obtained at 18°C by sitting drop vapor diffusion methods crystal structure of the SENP7 catalytic domain at a resolution of 2.4 A
Homo sapiens
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
C745K
mutation does not alter deconjugation rates in comparison with wild-type SENP7
Homo sapiens
F709W
end point deconjugation reactions using di-SUMO2/3 or poly-SUMO2/3 reveales rates equivalent to or slightly greater than wild-type SENP7. Mutation results in a 2fold higher activity when di-SUMO2 deconjugation rates are measured
Homo sapiens
V713E
mutation elicits 65-fold reduction in deconjugation rate compared to wild-type activity
Homo sapiens
V713E/C745K
C745K substitution partially rescues defects observed for SENP7-V713E when present as a double point substitution (SENP7-V713E/C745K)
Homo sapiens
Purification (Commentary) (protein specific)
Commentary
Organism
-
Homo sapiens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(SUMO-3)-Ran GTPase-activating protein 1 conjugate + H2O
SENP6 and SENP7 prefer SUMO2 or SUMO3 in deconjugation reactions with rates comparable with those catalyzed by SENP2. In contrast to SENP2, SENP6 and SENP7 are less able to deconjugate SUMO1-RanGAP1, and products are only detected at enzyme concentrations 100 x higher than that observed for reactions containing SENP2
715494
Homo sapiens
SUMO-3 + Ran GTPase-activating protein 1
-
-
-
?
di-SUMO-2 + H2O
-
715494
Homo sapiens
2 SUMO-2
-
-
-
?
di-SUMO-3 + H2O
-
715494
Homo sapiens
2 SUMO-3
-
-
-
?
poly-SUMO-2 + H2O
-
715494
Homo sapiens
?
-
-
-
?
poly-SUMO-3 + H2O
-
715494
Homo sapiens
?
-
-
-
?
SUMO-1 precursor + H2O
SENP7 exhibits lower rates for processing pre-SUMO-1, pre-SUMO-2, or pre-SUMO-3 in comparison with SENP2
715494
Homo sapiens
SUMO-1 + His-Ser-Thr-Val
-
-
-
?
SUMO-2 precursor + H2O
SENP7 exhibits lower rates for processing pre-SUMO1, pre-SUMO-2, or pre-SUMO-3 in comparison with SENP2
715494
Homo sapiens
SUMO-2 + Val-Tyr
-
-
-
?
SUMO-3 precursor + H2O
SENP7 exhibits lower rates for processing pre-SUMO1, pre-SUMO-2, or pre-SUMO-3 in comparison with SENP2
715494
Homo sapiens
SUMO-3 + Val-Pro-Glu-Ser-Ser-Leu-Ala-Gly-His-Ser-Phe
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
23
-
assay at
Homo sapiens
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Homo sapiens
Other publictions for EC 3.4.22.B75
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
731599
Gonzalez-Prieto
c-Myc is targeted to the prote ...
Homo sapiens
Cell Cycle
14
1859-1872
2015
-
-
-
-
1
-
-
-
1
-
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1
-
1
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5
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2
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1
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1
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1
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5
-
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2
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-
-
-
-
-
4
4
-
-
-
731614
Romeo
The SENP7 SUMO-protease presen ...
Mus musculus
Cell Rep.
10
771-782
2015
-
-
1
-
1
-
-
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1
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2
-
2
-
-
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1
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2
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1
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1
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1
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2
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1
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2
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-
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-
3
3
-
-
-
732884
Alegre
Structural insights into the S ...
Homo sapiens
Protein Sci.
23
433-441
2014
-
-
-
-
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-
-
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1
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1
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-
-
1
-
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-
-
-
-
-
2
2
-
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-
731715
Garvin
The deSUMOylase SENP7 promotes ...
Homo sapiens
EMBO Rep.
14
975-983
2013
-
-
1
-
2
-
-
-
1
-
-
2
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1
-
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-
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3
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1
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3
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3
3
-
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732566
Maison
The SUMO protease SENP7 is a c ...
Mus musculus
Nat. Struct. Mol. Biol.
19
458-460
2012
-
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1
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2
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1
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1
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2
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2
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1
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1
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1
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1
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2
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1
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2
2
-
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732812
Bawa-Khalfe
Differential expression of SUM ...
Homo sapiens
Proc. Natl. Acad. Sci. USA
109
17466-17471
2012
-
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2
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1
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1
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1
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3
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3
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2
2
-
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-
714083
Shen
Characterization of SENP7, a S ...
Homo sapiens
Biochem. J.
421
223-230
2009
-
-
1
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-
-
-
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1
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1
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1
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1
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4
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1
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1
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1
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1
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1
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1
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4
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1
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1
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2
2
-
-
-
714081
Drag
Activity profiling of human de ...
Homo sapiens
Biochem. J.
409
461-469
2008
2
-
1
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1
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1
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1
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1
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1
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1
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3
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1
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1
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-
715494
Lima
Structure of the human SENP7 c ...
Homo sapiens
J. Biol. Chem.
283
32045-32055
2008
-
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1
4
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1
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1
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8
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1
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1
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1
4
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8
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