BRENDA - Enzyme Database show
show all sequences of 3.4.22.B75

Characterization of SENP7, a SUMO-2/3-specific isopeptidase

Shen, L.N.; Geoffroy, M.C.; Jaffray, E.G.; Hay, R.T.; Biochem. J. 421, 223-230 (2009)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Homo sapiens
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
nucleoplasm
-
Homo sapiens
5654
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
poly-SUMO-2 + H2O
Homo sapiens
the C-terminal catalytic domain of SENP7 efficiently depolymerized poly-SUMO-2 chains but has undetectable activity against poly-SUMO-1 chains
?
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
-
-
Purification (Commentary)
Commentary
Organism
-
Homo sapiens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(di-SUMO-2)-Ran GTPase-activating protein 1 conjugate + H2O
SENP7 displays isopeptidase activity against di-SUMO-2- and SUMO-2-modified Ran GTPase-activating protein 1 but has limited activity against SUMO-1-modified Ran GTPase-activating protein 1
714083
Homo sapiens
?
-
-
-
?
(SUMO-2)-Ran GTPase-activating protein 1 conjugate + H2O
SENP7 displays isopeptidase activity against di-SUMO-2- and SUMO-2-modified Ran GTPase-activating protein 1 but has limited activity against SUMO-1-modified Ran GTPase-activating protein 1
714083
Homo sapiens
?
-
-
-
?
additional information
SENP7 has a restricted substrate specificity, being unable to process SUMO precursors and displaying paralogue-specific isopeptidase activity
714083
Homo sapiens
?
-
-
-
-
poly-SUMO-2 + H2O
the C-terminal catalytic domain of SENP7 efficiently depolymerized poly-SUMO-2 chains but has undetectable activity against poly-SUMO-1 chains
714083
Homo sapiens
?
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Homo sapiens
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Homo sapiens
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Homo sapiens
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
nucleoplasm
-
Homo sapiens
5654
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
poly-SUMO-2 + H2O
Homo sapiens
the C-terminal catalytic domain of SENP7 efficiently depolymerized poly-SUMO-2 chains but has undetectable activity against poly-SUMO-1 chains
?
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Homo sapiens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(di-SUMO-2)-Ran GTPase-activating protein 1 conjugate + H2O
SENP7 displays isopeptidase activity against di-SUMO-2- and SUMO-2-modified Ran GTPase-activating protein 1 but has limited activity against SUMO-1-modified Ran GTPase-activating protein 1
714083
Homo sapiens
?
-
-
-
?
(SUMO-2)-Ran GTPase-activating protein 1 conjugate + H2O
SENP7 displays isopeptidase activity against di-SUMO-2- and SUMO-2-modified Ran GTPase-activating protein 1 but has limited activity against SUMO-1-modified Ran GTPase-activating protein 1
714083
Homo sapiens
?
-
-
-
?
additional information
SENP7 has a restricted substrate specificity, being unable to process SUMO precursors and displaying paralogue-specific isopeptidase activity
714083
Homo sapiens
?
-
-
-
-
poly-SUMO-2 + H2O
the C-terminal catalytic domain of SENP7 efficiently depolymerized poly-SUMO-2 chains but has undetectable activity against poly-SUMO-1 chains
714083
Homo sapiens
?
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Homo sapiens
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Homo sapiens
General Information
General Information
Commentary
Organism
malfunction
small interfering RNA-mediated ablation of SENP7 expression leads to the accumulation of high-molecular-mass SUMO-2 species and to the accumulation of promyelocytic leukaemia protein in subnuclear bodies
Homo sapiens
physiological function
SENP7 acts as a SUMO-2/3-specific protease that is likely to regulate the metabolism of poly-SUMO-2/3 rather than SUMO-1 conjugation in vivo
Homo sapiens
General Information (protein specific)
General Information
Commentary
Organism
malfunction
small interfering RNA-mediated ablation of SENP7 expression leads to the accumulation of high-molecular-mass SUMO-2 species and to the accumulation of promyelocytic leukaemia protein in subnuclear bodies
Homo sapiens
physiological function
SENP7 acts as a SUMO-2/3-specific protease that is likely to regulate the metabolism of poly-SUMO-2/3 rather than SUMO-1 conjugation in vivo
Homo sapiens
Other publictions for EC 3.4.22.B75
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
731599
Gonzalez-Prieto
c-Myc is targeted to the prote ...
Homo sapiens
Cell Cycle
14
1859-1872
2015
-
-
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1
-
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1
-
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1
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1
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2
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1
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1
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5
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2
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-
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4
4
-
-
-
731614
Romeo
The SENP7 SUMO-protease presen ...
Mus musculus
Cell Rep.
10
771-782
2015
-
-
1
-
1
-
-
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1
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2
-
2
-
-
-
-
-
1
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2
-
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1
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1
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1
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2
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1
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2
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3
3
-
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732884
Alegre
Structural insights into the S ...
Homo sapiens
Protein Sci.
23
433-441
2014
-
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1
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1
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2
2
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731715
Garvin
The deSUMOylase SENP7 promotes ...
Homo sapiens
EMBO Rep.
14
975-983
2013
-
-
1
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2
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1
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2
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1
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3
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3
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3
3
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732566
Maison
The SUMO protease SENP7 is a c ...
Mus musculus
Nat. Struct. Mol. Biol.
19
458-460
2012
-
-
1
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2
-
-
-
1
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1
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2
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2
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1
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1
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2
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1
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1
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1
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2
2
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732812
Bawa-Khalfe
Differential expression of SUM ...
Homo sapiens
Proc. Natl. Acad. Sci. USA
109
17466-17471
2012
-
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2
-
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1
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1
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1
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3
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3
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2
2
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714083
Shen
Characterization of SENP7, a S ...
Homo sapiens
Biochem. J.
421
223-230
2009
-
-
1
-
-
-
-
-
1
-
-
1
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1
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1
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4
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1
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1
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1
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4
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1
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1
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2
2
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714081
Drag
Activity profiling of human de ...
Homo sapiens
Biochem. J.
409
461-469
2008
2
-
1
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1
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3
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1
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1
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715494
Lima
Structure of the human SENP7 c ...
Homo sapiens
J. Biol. Chem.
283
32045-32055
2008
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1
4
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1
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8
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4
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