BRENDA - Enzyme Database show
show all sequences of 3.4.22.B74

Activity profiling of human deSUMOylating enzymes (SENPs) with synthetic substrates suggests an unexpected specificity of two newly characterized members of the family

Drag, M.; Mikolajczyk, J.; Krishnakumar, I.M.; Huang, Z.; Salvesen, G.S.; Biochem. J. 409, 461-469 (2008)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
truncated SUMO-2
whereas truncated SUMO-2 and -3 can substantially enhance SENP6 activity, neither truncated SUMO-1 nor truncated Nedd8 nor ubiquitin has this ability
Homo sapiens
truncated SUMO-3
whereas truncated SUMO-2 and -3 can substantially enhance SENP6 activity, neither truncated SUMO-1 nor truncated Nedd8 nor ubiquitin has this ability
Homo sapiens
Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Homo sapiens
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
keratinocyte
-
Homo sapiens
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-LRGG-7-amido-4-trifluoromethylcoumarin + H2O
SENP6 prefers the LRGG sequence, residues typical of Nedd8 or ubiquitin in the P3 and P4 positions
714081
Homo sapiens
acetyl-LRGG + 7-amino-4-trifluoromethylcoumarin
-
-
-
?
acetyl-QTGG-7-amido-4-trifluoromethylcoumarin + H2O
very low activity
714081
Homo sapiens
acetyl-QTGG + 7-amino-4-trifluoromethylcoumarin
-
-
-
?
additional information
SENP6 reveals no detectable endopeptidase activity on the precursors of ubiquitin, Nedd8 and ISG15
714081
Homo sapiens
?
-
-
-
-
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Homo sapiens
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Homo sapiens
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
truncated SUMO-2
whereas truncated SUMO-2 and -3 can substantially enhance SENP6 activity, neither truncated SUMO-1 nor truncated Nedd8 nor ubiquitin has this ability
Homo sapiens
truncated SUMO-3
whereas truncated SUMO-2 and -3 can substantially enhance SENP6 activity, neither truncated SUMO-1 nor truncated Nedd8 nor ubiquitin has this ability
Homo sapiens
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Homo sapiens
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
keratinocyte
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-LRGG-7-amido-4-trifluoromethylcoumarin + H2O
SENP6 prefers the LRGG sequence, residues typical of Nedd8 or ubiquitin in the P3 and P4 positions
714081
Homo sapiens
acetyl-LRGG + 7-amino-4-trifluoromethylcoumarin
-
-
-
?
acetyl-QTGG-7-amido-4-trifluoromethylcoumarin + H2O
very low activity
714081
Homo sapiens
acetyl-QTGG + 7-amino-4-trifluoromethylcoumarin
-
-
-
?
additional information
SENP6 reveals no detectable endopeptidase activity on the precursors of ubiquitin, Nedd8 and ISG15
714081
Homo sapiens
?
-
-
-
-
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Homo sapiens
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Homo sapiens
Other publictions for EC 3.4.22.B74
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
732884
Alegre
Structural insights into the S ...
Homo sapiens
Protein Sci.
23
433-441
2014
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
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-
1
-
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-
-
-
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-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
715706
Mukhopadhyay
The SUMO protease SENP6 is ess ...
Homo sapiens
J. Cell Biol.
188
681-692
2010
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
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-
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-
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-
2
2
-
-
-
716169
Hattersley
The SUMO protease SENP6 is a d ...
Homo sapiens
Mol. Biol. Cell
22
78-90
2010
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
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-
-
-
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1
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-
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-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
714081
Drag
Activity profiling of human de ...
Homo sapiens
Biochem. J.
409
461-469
2008
2
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
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3
-
1
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1
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2
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1
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1
-
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3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
715494
Lima
Structure of the human SENP7 c ...
Homo sapiens
J. Biol. Chem.
283
32045-32055
2008
-
-
-
-
-
-
-
-
-
-
-
-
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1
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1
-
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8
-
1
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1
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1
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8
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1
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1
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715444
Kim
A new SUMO-1-specific protease ...
Homo sapiens
J. Biol. Chem.
275
14102-14106
2000
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1
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1
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1
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4
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9
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3
1
1
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1
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1
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1
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1
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9
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3
1
1
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1
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