BRENDA - Enzyme Database
show all sequences of 3.4.22.B74

A new SUMO-1-specific protease, SUSP1, that is highly expressed in reproductive organs

Kim, K.I.; Baek, S.H.; Jeon, Y.J.; Nishimori, S.; Suzuki, T.; Uchida, S.; Shimbara, N.; Saitoh, H.; Tanaka, K.; Chung, C.H.; J. Biol. Chem. 275, 14102-14106 (2000)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Homo sapiens
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
SUSP1 is exclusively localized to the cytoplasm of NIH3T3 and HeLa cells
Homo sapiens
5737
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
126116
-
x * 126116, calculated from sequence
Homo sapiens
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
Q9GZR1
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
brain
little activity
Homo sapiens
-
colon
reproductive organs, such as testis, ovary, and prostate, contain much higher amounts of SUSP1 mRNA than colon and peripheral blood leukocyte
Homo sapiens
-
HeLa cell
-
Homo sapiens
-
leukocyte
reproductive organs, such as testis, ovary, and prostate, contain much higher amounts of SUSP1 mRNA than colon and peripheral blood leukocyte
Homo sapiens
-
additional information
no or little SUSP1 mRNA in liver, lung, kidney, pancreas, spleen, thymus, heart, and skeletal muscle
Homo sapiens
-
ovary
reproductive organs, such as testis, ovary, and prostate, contain much higher amounts of SUSP1 mRNA than colon and peripheral blood leukocyte
Homo sapiens
-
prostate
reproductive organs, such as testis, ovary, and prostate, contain much higher amounts of SUSP1 mRNA than colon and peripheral blood leukocyte
Homo sapiens
-
testis
reproductive organs, such as testis, ovary, and prostate, contain much higher amounts of SUSP1 mRNA than colon and peripheral blood leukocyte
Homo sapiens
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(SUMO-1)-beta-galactosidase conjugate + H2O
SUSP1 expressed in Escherichia coli cells efficiently releases SUMO-1 from SUMO-1-beta-galactosidase fusion but not from other ubiquitin-like protein fusions, including Smt3-beta-galactosidase
715444
Homo sapiens
SUMO-1 + beta-galactosidase
-
-
-
?
additional information
SUSP1 expressed in Escherichia coli cells efficiently releases SUMO-1 from SUMO-1-beta-galactosidase fusion but not from other ubiquitin-like protein fusions, including Smt3zb-galactosidase, suggesting its role in the generation of matured SUMO-1 specifically from its precursors
715444
Homo sapiens
?
-
-
-
-
SUMO-1 precursor + H2O
-
715444
Homo sapiens
SUMO-1 + His-Ser-Thr-Val
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 126116, calculated from sequence
Homo sapiens
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Homo sapiens
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
-
assay at
Homo sapiens
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Homo sapiens
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
SUSP1 is exclusively localized to the cytoplasm of NIH3T3 and HeLa cells
Homo sapiens
5737
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
126116
-
x * 126116, calculated from sequence
Homo sapiens
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
brain
little activity
Homo sapiens
-
colon
reproductive organs, such as testis, ovary, and prostate, contain much higher amounts of SUSP1 mRNA than colon and peripheral blood leukocyte
Homo sapiens
-
HeLa cell
-
Homo sapiens
-
leukocyte
reproductive organs, such as testis, ovary, and prostate, contain much higher amounts of SUSP1 mRNA than colon and peripheral blood leukocyte
Homo sapiens
-
additional information
no or little SUSP1 mRNA in liver, lung, kidney, pancreas, spleen, thymus, heart, and skeletal muscle
Homo sapiens
-
ovary
reproductive organs, such as testis, ovary, and prostate, contain much higher amounts of SUSP1 mRNA than colon and peripheral blood leukocyte
Homo sapiens
-
prostate
reproductive organs, such as testis, ovary, and prostate, contain much higher amounts of SUSP1 mRNA than colon and peripheral blood leukocyte
Homo sapiens
-
testis
reproductive organs, such as testis, ovary, and prostate, contain much higher amounts of SUSP1 mRNA than colon and peripheral blood leukocyte
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(SUMO-1)-beta-galactosidase conjugate + H2O
SUSP1 expressed in Escherichia coli cells efficiently releases SUMO-1 from SUMO-1-beta-galactosidase fusion but not from other ubiquitin-like protein fusions, including Smt3-beta-galactosidase
715444
Homo sapiens
SUMO-1 + beta-galactosidase
-
-
-
?
additional information
SUSP1 expressed in Escherichia coli cells efficiently releases SUMO-1 from SUMO-1-beta-galactosidase fusion but not from other ubiquitin-like protein fusions, including Smt3zb-galactosidase, suggesting its role in the generation of matured SUMO-1 specifically from its precursors
715444
Homo sapiens
?
-
-
-
-
SUMO-1 precursor + H2O
-
715444
Homo sapiens
SUMO-1 + His-Ser-Thr-Val
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 126116, calculated from sequence
Homo sapiens
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Homo sapiens
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
-
assay at
Homo sapiens
Other publictions for EC 3.4.22.B74
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
732884
Alegre
Structural insights into the S ...
Homo sapiens
Protein Sci.
23
433-441
2014
-
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1
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1
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2
2
-
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-
715706
Mukhopadhyay
The SUMO protease SENP6 is ess ...
Homo sapiens
J. Cell Biol.
188
681-692
2010
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1
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2
2
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716169
Hattersley
The SUMO protease SENP6 is a d ...
Homo sapiens
Mol. Biol. Cell
22
78-90
2010
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1
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1
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1
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2
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1
1
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714081
Drag
Activity profiling of human de ...
Homo sapiens
Biochem. J.
409
461-469
2008
2
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1
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1
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3
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1
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2
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1
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1
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3
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1
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1
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715494
Lima
Structure of the human SENP7 c ...
Homo sapiens
J. Biol. Chem.
283
32045-32055
2008
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1
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1
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8
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1
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1
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1
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8
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1
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1
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715444
Kim
A new SUMO-1-specific protease ...
Homo sapiens
J. Biol. Chem.
275
14102-14106
2000
-
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1
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-
-
-
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1
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1
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4
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9
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3
1
1
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1
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1
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1
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1
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9
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3
1
1
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1
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