Cloned (Comment) | Organism |
---|---|
overexpression of a catalytically inactive/dominant negative Cys-to-Ser SENP1 mutant | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a SENP1 catalytically inactive/dominant negative Cys-to-Ser SENP1 mutant or a SENP1 shRNA construct, enzyme SENP1 silencing by expression of siRNA | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | SENP1 is primarily localized in the nucleus | Homo sapiens | 5634 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | the enzyme SUMO protease removes SUMO conjugate from proteins | ? | - |
? | |
Pin1 + H2O | Homo sapiens | Pin1 is SUMOylated on Lys6 in the WW domain and on Lys63 in the PPIase domain, deSUMOlation by SENP1 | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
breast cancer cell | enzyme SENP1 levels are positively correlated with substrate Pin1 levels in human breast cancer specimens | Homo sapiens | - |
carcinoma cell | the enzyme expression is deregulated in cancers | Homo sapiens | - |
MCF-10A cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme SUMO protease removes SUMO conjugate from proteins | Homo sapiens | ? | - |
? | |
Pin1 + H2O | Pin1 is SUMOylated on Lys6 in the WW domain and on Lys63 in the PPIase domain, deSUMOlation by SENP1 | Homo sapiens | ? | - |
? | |
Pin1 + H2O | Pin1 is SUMOylated on Lys6 in the WW domain and on Lys63 in the PPIase domain, deSUMOlation by SENP1. SENP1 is more efficient in deSUMOylating Pin1 than SENP2, whereas SENP3 is barely active | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SENP | - |
Homo sapiens |
SENP1 | - |
Homo sapiens |
SUMO protease | - |
Homo sapiens |
Organism | Comment | Expression |
---|---|---|
Homo sapiens | enzyme SENP1 silencing by expression of siRNA | down |
General Information | Comment | Organism |
---|---|---|
malfunction | inhibition of endogenous enzyme SENP1 via the SENP1 dominant-negative mutant or shRNA-induced Pin1 SUMOylation in vivo. Knockdown of endogenous enzyme SENP1 expression also reduces Pin1-mediated cyclin D1 activation and again the enhanced ability of Pin1K6/63R to activate the cyclin D1 promoter is not reduced by enzyme SENP1 | Homo sapiens |
physiological function | Pin1 is SUMOylated on Lys6 in the WW domain and on Lys63 in the PPIase domain. Pin1 SUMOylation inhibits its protein activity and oncogenic function, the enzyme removes the SUMO conjugates and activates Pin1 promoting the ability of Pin1 to induce centrosome amplification and cell transformation, overview. Enzyme SENP1 also increases Pin1 protein stability in cell cultures, and Pin1 levels are positively correlated with SENP1 levels in human breast cancer specimens. Pin1 SUMOylation on Lys6/63 as a distinct mechanism to inhibit its activity and function but also identify a critical role for SENP1-mediated deSUMOylation in promoting Pin1 function during tumorigenesis | Homo sapiens |