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Literature summary for 3.4.22.B70 extracted from

  • Xu, Z.; Au, S.W.
    Mapping residues of SUMO precursors essential in differential maturation by SUMO-specific protease, SENP1 (2005), Biochem. J., 386, 325-330.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.22.B70

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
SUMO-1 precursor + H2O Homo sapiens cleavage of the -Gly-Gly-/-His-Ser-Thr-Val bond. The maturation reaction is the first committed step for subsequent sumoylation SUMO-1 + His-Ser-Thr-Val
-
 ?
SUMO-2 precursor + H2O Homo sapiens cleavage of the -Gly-Gly-/-Val-Tyr. The maturation reaction is the first committed step for subsequent sumoylation SUMO-2 + Val-Tyr
-
 ?
SUMO-3 precursor + H2O Homo sapiens cleavage of the -Gly-Gly-/-Val-Pro-Glu-Ser-Ser-Leu-Ala-Gly-His-Ser-Phe bond. The maturation reaction is the first committed step for subsequent sumoylation SUMO-3 + Val-Pro-Glu-Ser-Ser-Leu-Ala-Gly-His-Ser-Phe
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9P0U3
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Homo sapiens
-
ovary
-
 Homo sapiens
-
pancreas
-
 Homo sapiens
-
small intestine
-
 Homo sapiens
-
spleen
-
 Homo sapiens
-
testis highest expression Homo sapiens
-
thymus
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
SUMO-1 precursor + H2O cleavage of the -Gly-Gly-/-His-Ser-Thr-Val bond. The maturation reaction is the first committed step for subsequent sumoylation Homo sapiens SUMO-1 + His-Ser-Thr-Val
-
 ?
SUMO-1 precursor + H2O SENP1 is capable of processing all SUMO-1, -2 and -3 in vitro but with different efficiencies. Cleavage of the -Gly-Gly-/-His-Ser-Thr-Val bond. Over 90% of SUMO-1 is hydrolysed Homo sapiens SUMO-1 + His-Ser-Thr-Val
-
 ?
SUMO-2 precursor + H2O cleavage of the -Gly-Gly-/-Val-Tyr. The maturation reaction is the first committed step for subsequent sumoylation Homo sapiens SUMO-2 + Val-Tyr
-
 ?
SUMO-2 precursor + H2O SENP1 is capable of processing all SUMO-1, -2 and -3 in vitro but with different efficiencies. Over 90% of SUMO-2 is hydrolysed. Cleavage of the -Gly-Gly-/-Val-Tyr Homo sapiens SUMO-2 + Val-Tyr
-
 ?
SUMO-3 precursor + H2O cleavage of the -Gly-Gly-/-Val-Pro-Glu-Ser-Ser-Leu-Ala-Gly-His-Ser-Phe bond. The maturation reaction is the first committed step for subsequent sumoylation Homo sapiens SUMO-3 + Val-Pro-Glu-Ser-Ser-Leu-Ala-Gly-His-Ser-Phe
-
 ?
SUMO-3 precursor + H2O SENP1 is capable of processing all SUMO-1, -2 and -3 in vitro but with different efficiencies. 50% of SUMO-3 is hydrolysed. Cleavage of the -Gly-Gly-/-Val-Pro-Glu-Ser-Ser-Leu-Ala-Gly-His-Ser-Phe bond Homo sapiens SUMO-3 + Val-Pro-Glu-Ser-Ser-Leu-Ala-Gly-His-Ser-Phe
-
 ?

Synonyms

Synonyms Comment Organism
SENP1
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens