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Literature summary for 3.4.22.B65 extracted from

  • Mason, R.W.; Bergman, C.A.; Lu, G.; Frenck Holbrook, J.; Sol-Church, K.
    Expression and characterization of cathepsin P (2004), Biochem. J., 378, 657-663.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.22.B65

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol activation Mus musculus
hyaluronate
-
 Mus musculus
Na2SO4
-
 Mus musculus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
biotin-(6-hexanamido-hexanoate)2-Tyr-Ala-CHN2
-
 Mus musculus
E-64
-
 Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24000
-
 x * 31000, x * 35000, x * 38000, SDS-PAGE of recombinant zymogen, x * 24000, x * 27000, SDS-PAGE of mature protein Mus musculus
27000
-
 x * 31000, x * 35000, x * 38000, SDS-PAGE of recombinant zymogen, x * 24000, x * 27000, SDS-PAGE of mature protein Mus musculus
31000
-
 x * 31000, x * 35000, x * 38000, SDS-PAGE of recombinant zymogen, x * 24000, x * 27000, SDS-PAGE of mature protein Mus musculus
35000
-
 x * 31000, x * 35000, x * 38000, SDS-PAGE of recombinant zymogen, x * 24000, x * 27000, SDS-PAGE of mature protein Mus musculus
38000
-
 x * 31000, x * 35000, x * 38000, SDS-PAGE of recombinant zymogen, x * 24000, x * 27000, SDS-PAGE of mature protein Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus Q9R014
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein treatment of recombinant protein with PNGase F results in a single protein band of 29000 Da in SDS-PAGE for the zymogen and of 22000 Da for the autoactivated protein Mus musculus
proteolytic modification the inactive zymogen can be activated by proteinase K, chymotrypsin or pancreatic elastase at neutral pH. Cathepsin P can be autoactivated at neutral pH, but not at acidic pH Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7-(methoxycoumarin-4-yl)acetyl-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2 + H2O
-
 Mus musculus 7-(methoxycoumarin-4-yl)acetyl-Pro-Leu-Gly + Leu-Dap(Dnp)-Ala-Arg-NH2
-
 ?
azocasein + H2O
-
 Mus musculus ?
-
 ?
additional information no substrates: Z-Phe-Arg-NHMec, Z-Arg-Arg-NHMec, Arg-NHMec, Bz-Phe-Val-Arg-NHMec, succinyl-Ala-Ala-Phe-NHMec, Z-Val-Ala-Asp-NHMec, bradykinin, somatostatin, Fmoc-Tyr-Ala-OH, Z-Gly-Gly-OH, Z-Leu-Tyr-OH, Dnp-Pro-Leu-Gly-Leu-Trp-Ala-D-Arg-NH2, Mca-Pro-Leu-Ala-Gln-Ala-Val-Dpa-Arg-Ser-Ser-Ser-Arg-NH2 or Dnp-Pro-cyclohexyl-Ala-Gly-Cys(Me)-His-Ala-Lys(Nma)NH2 Mus musculus ?
-
 ?
transferrin + H2O
-
 Mus musculus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 31000, x * 35000, x * 38000, SDS-PAGE of recombinant zymogen, x * 24000, x * 27000, SDS-PAGE of mature protein Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7.5 substrates azocasein and transferrin Mus musculus
7
-
 substrate 7-(methoxycoumarin-4-yl)acetyl-Pro-Leu-Gly-Leu-Dnp(Dpa)-Ala-Arg-NH2 Mus musculus

pH Range

pH Minimum pH Maximum Comment Organism
5
-
 little activity below Mus musculus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.002
-
 E-64 37°C, presence of 1.5 M NaSO4 Mus musculus