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Literature summary for 3.4.22.B62 extracted from

  • Celias, D.P.; Corvo, I.; Silvane, L.; Tort, J.F.; Chiapello, L.S.; Fresno, M.; Arranz, A.; Motran, C.C.; Cervi, L.
    Cathepsin L3 from Fasciola hepatica induces NLRP3 inflammasome alternative activation in murine dendritic cells (2019), Front. Immunol., 10, 552 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.22.B62

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular the enzyme is secreted Fasciola hepatica
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-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Collagen + H2O Fasciola hepatica
-
 ?
-
 ?
pro-interleukin-1beta + H2O Fasciola hepatica
-
 interleukin-1beta + interleukin-1beta propeptide
-
 ?

Organism

Organism UniProt Comment Textmining
Fasciola hepatica B3TM67
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-

Source Tissue

Source Tissue Comment Organism Textmining
additional information cathepsin L3 (FhCL3) is expressed mainly in the juvenile or invasive stage Fasciola hepatica
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Collagen + H2O
-
 Fasciola hepatica ?
-
 ?
pro-interleukin-1beta + H2O
-
 Fasciola hepatica interleukin-1beta + interleukin-1beta propeptide
-
 ?

Synonyms

Synonyms Comment Organism
FhCL3
-
 Fasciola hepatica

General Information

General Information Comment Organism
physiological function in the case of the helminth trematode Fasciola hepatica, the secretion of different cathepsin L cysteine peptidases (FhCL) is crucial for the parasite survival. Among these enzymes, cathepsin L3 (FhCL3) is expressed mainly in the juvenile or invasive stage. The ability of FhCL3 to digest collagen is critical for intestinal tissue invasion during juvenile larvae migration. FhCL3 induces a non-canonical inflammasome activation in dendritic cells (DCs) of C57BL/6 mice, leading to interleukin (IL)-1beta and IL-18 production without a previous microbial priming. This activation is depending on the cysteine protease activity of FhCL3 and the NLRP3 receptor, but independent of caspase activation. FhCL3 is internalized by DCs, promoting pro-IL-1beta cleavage to its mature and biologically active form IL-1beta, which is released to the extracellular environment. The FhCL3-induced NLRP3 inflammasome activation conditions DCs to promote a singular adaptive immune response, characterized by increased production of IFN-gamma and IL-13. Due to the poor colocalization of FhCL3 with endosomes or lysosomes, the mechanism of endocytosis could be different from that used by FhCL1. The lack of involvement of NF-kkappaB in FhCL3-induced DCs activation, suggests that other/s transcription factor might be involved in the synthesis of pro-IL-1beta Fasciola hepatica