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Literature summary for 3.4.22.B62 extracted from

  • Zawistowska-Deniziak, A.; Wasyl, K.; Norbury, L.J.; Wesolowska, A.; Bien, J.; Grodzik, M.; Wisniewski, M.; Baska, P.; Wedrychowicz, H.
    Characterization and differential expression of cathepsin L3 alleles from Fasciola hepatica (2013), Mol. Biochem. Parasitol., 190, 27-37.
    View publication on PubMed
Search for more literature for 3.4.22.B62

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 and Pichia pastoris X33 cells Fasciola hepatica

Inhibitors

Inhibitors Comment Organism Structure
E-64
-
 Fasciola hepatica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38080
-
 x * 38080, isoform CL3-1, calculated from amino acid sequence Fasciola hepatica
38280
-
 x * 38280, isoform CL3-2, calculated from amino acid sequence Fasciola hepatica
46000
-
 x * 46000, recombinant proenzyme, SDS-PAGE Fasciola hepatica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Collagen + H2O Fasciola hepatica
-
 ?
-
 ?
human immumglobulin G + H2O Fasciola hepatica partial cleavage ?
-
 ?

Organism

Organism UniProt Comment Textmining
Fasciola hepatica B9VXS1 isoform CL3-2
-
Fasciola hepatica B9VXS2 isoform CL3-1
-

Purification (Commentary)

Purification (Comment) Organism
nickel affinity column chromatography Fasciola hepatica

Source Tissue

Source Tissue Comment Organism Textmining
juvenile newly excysted Fasciola hepatica
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Collagen + H2O
-
 Fasciola hepatica ?
-
 ?
human immumglobulin G + H2O partial cleavage Fasciola hepatica ?
-
 ?
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin + H2O
-
 Fasciola hepatica tosyl-Gly-Pro-Arg + 7-amino-4-methylcoumarin
-
 ?

Subunits

Subunits Comment Organism
? x * 38080, isoform CL3-1, calculated from amino acid sequence Fasciola hepatica
? x * 38280, isoform CL3-2, calculated from amino acid sequence Fasciola hepatica
? x * 46000, recombinant proenzyme, SDS-PAGE Fasciola hepatica

Synonyms

Synonyms Comment Organism
CL3
-
 Fasciola hepatica
CL3-1 isoform with high expression Fasciola hepatica
CL3-2 isoform with low expression Fasciola hepatica

Expression

Organism Comment Expression
Fasciola hepatica maximum expression level is observed in newly excysted juveniles immediately after excystment up