Literature summary for 3.4.22.B60 extracted from

Martinez-Sernandez, V.; Perteguer, M.J.; Hernandez-Gonzalez, A.; Mezo, M.; Gonzalez-Warleta, M.; Orbegozo-Medina, R.A.; Romaris, F.; Paniagua, E.; Garate, T.; Ubeira, F.M.
Comparison of recombinant cathepsins L1, L2, and L5 as ELISA targets for serodiagnosis of bovine and ovine fascioliasis (2018), Parasitol. Res., 117, 1521-1534 .

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Application

Application	Comment	Organism
diagnostics	analysis of the diagnostic values of the three different clades of cathepsin Ls, FhCL1, FhCL2, and FhCL5, from adult flukes in an ELISA, test of sera from sheep and cattle naturally infected with Fasciola hepatica, assessment of cross-reactive antibodies, overview. For sheep sera, the sensitivity is 100% for the three rFhpCLs, while for cattle sera, the highest sensitivity is obtained using rFhpCL2 (97%), being equal for both rFhpCL1 and rFhpCL5 (87.9%), after adjusting cut-offs for maximum specificity	Fasciola hepatica

Cloned(Commentary)

Cloned (Comment)	Organism
gene FhpCL2, DNA and amino acid sequence determination and analysis, sequence comparisons of rFhpCLs, recombinant expression of His-tagged enzyme in Escherichia coli	Fasciola hepatica

Organism

Organism	UniProt	Comment	Textmining
Fasciola hepatica	A0A220T1Z2	procathepsin L2	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant solubilized and refolded His-tagged enzyme from Escherichia coli by nickel affinity chromatography	Fasciola hepatica

Renatured (Commentary)

Renatured (Comment)	Organism
recombinant His-tagged enzyme from Escherichia coli inclusion bodies, solubilization by 8 M urea, followed by dilution, dialysis, and ultrafiltration	Fasciola hepatica

Synonyms

Synonyms	Comment	Organism
FhpCL2	-	Fasciola hepatica

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Fasciola hepatica

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	assay at	Fasciola hepatica

General Information

General Information	Comment	Organism
evolution	adult flukes produce three different clades of cathepsin Ls: FhCL1, FhCL2, and FhCL5	Fasciola hepatica

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Release 2023.1 (January 2023)