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Literature summary for 3.4.22.B60 extracted from

  • Robinson, M.W.; Corvo, I.; Jones, P.M.; George, A.M.; Padula, M.P.; To, J.; Cancela, M.; Rinaldi, G.; Tort, J.F.; Roche, L.; Dalton, J.P.
    Collagenolytic activities of the major secreted cathepsin L peptidases involved in the virulence of the helminth pathogen, Fasciola hepatica (2011), PLoS Negl. Trop. Dis., 5, e1012.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.22.B60

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia angusta Fasciola hepatica

Inhibitors

Inhibitors Comment Organism Structure
cathepsin K inhibitor II i.e. Z-LNHNHCONHNHLF-Boc Fasciola hepatica
Z-Phe-Ala-CHN2
-
 Fasciola hepatica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00153
-
 Z-VVR-7-amido-4-methylcoumarin in 100 mM sodium phosphate buffer (pH 6.0) containing 1 mM dithiothreitol and 1 mM EDTA, at 37°C Fasciola hepatica
0.00213
-
 Z-Leu-Arg-NHMe in 100 mM sodium phosphate buffer (pH 6.0) containing 1 mM dithiothreitol and 1 mM EDTA, at 37°C Fasciola hepatica
0.0022
-
 Boc-VLK-7-amido-4-methylcoumarin in 100 mM sodium phosphate buffer (pH 6.0) containing 1 mM dithiothreitol and 1 mM EDTA, at 37°C Fasciola hepatica
0.0078
-
 Z-Phe-Arg-NHMe in 100 mM sodium phosphate buffer (pH 6.0) containing 1 mM dithiothreitol and 1 mM EDTA, at 37°C Fasciola hepatica
0.0094
-
 Boc-AGPR-7-amido-4-methylcoumarin in 100 mM sodium phosphate buffer (pH 6.0) containing 1 mM dithiothreitol and 1 mM EDTA, at 37°C Fasciola hepatica
0.01139
-
 Boc-VPR-7-amido-4-methylcoumarin in 100 mM sodium phosphate buffer (pH 6.0) containing 1 mM dithiothreitol and 1 mM EDTA, at 37°C Fasciola hepatica
0.0129
-
 tosyl-GPK-7-amido-4-methylcoumarin in 100 mM sodium phosphate buffer (pH 6.0) containing 1 mM dithiothreitol and 1 mM EDTA, at 37°C Fasciola hepatica
0.0139
-
 tosyl-GPR-7-amido-4-methylcoumarin in 100 mM sodium phosphate buffer (pH 6.0) containing 1 mM dithiothreitol and 1 mM EDTA, at 37°C Fasciola hepatica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24500
-
 x * 24500, mature enzyme, SDS-PAGE Fasciola hepatica
37000
-
 x * 37000, proenzyme, SDS-PAGE Fasciola hepatica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Boc-AGPR-7-amido-4-methylcoumarin + H2O Fasciola hepatica
-
 ?
-
 ?
Boc-VLK-7-amido-4-methylcoumarin + H2O Fasciola hepatica
-
 ?
-
 ?
Boc-VPR-7-amido-4-methylcoumarin + H2O Fasciola hepatica
-
 ?
-
 ?
Collagen type I + H2O Fasciola hepatica cathepsin L2 cleaves collagen type I at 43 sites within the alpha1 chain and 26 sites within the alpha2 chain ?
-
 ?
collagen type II + H2O Fasciola hepatica cathepsin L2 exhibits collagenase activity by cleaving at multiple sites within the alpha1 and alpha2 triple helix regions (Col domains) ?
-
 ?
additional information Fasciola hepatica cathepsin L2 readily cleaves substrates with Pro in the P2 position and peptide substrates mimicking the repeating Gly-Pro-Xaa motifs ?
-
 ?
additional information Fasciola hepatica the cathepsin L zymogen is autocatalytically activated and processed to mature enzyme by incubation for 2 h at 37°C in 0.1 M sodium citrate buffer (pH 5.0) containing 2 mM dithiothreitol and 2.5 mM EDTA ?
-
 ?
tosyl-GPK-7-amido-4-methylcoumarin + H2O Fasciola hepatica
-
 ?
-
 ?
tosyl-GPR-7-amido-4-methylcoumarin + H2O Fasciola hepatica
-
 ?
-
 ?
Z-Gly-Pro-Gly-Gly-Pro-Ala + H2O Fasciola hepatica
-
 Z-Gly-Pro + Gly-Gly + L-Pro-L-Ala
-
 ?
Z-Gly-Pro-Leu-Gly-Pro + H2O Fasciola hepatica
-
 Z-Gly-Pro + L-Leu + Gly + L-Pro
-
 ?
Z-Leu-Arg-NHMe + H2O Fasciola hepatica
-
 ?
-
 ?
Z-Phe-Arg-NHMe + H2O Fasciola hepatica
-
 ?
-
 ?
Z-VVR-7-amido-4-methylcoumarin + H2O Fasciola hepatica
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Fasciola hepatica
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA-agarose column chromatography Fasciola hepatica

Source Tissue

Source Tissue Comment Organism Textmining
larva liver migrating juvenile and mature bile duct parasites Fasciola hepatica
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Boc-AGPR-7-amido-4-methylcoumarin + H2O
-
 Fasciola hepatica ?
-
 ?
Boc-VLK-7-amido-4-methylcoumarin + H2O
-
 Fasciola hepatica ?
-
 ?
Boc-VPR-7-amido-4-methylcoumarin + H2O
-
 Fasciola hepatica ?
-
 ?
Collagen type I + H2O cathepsin L2 cleaves collagen type I at 43 sites within the alpha1 chain and 26 sites within the alpha2 chain Fasciola hepatica ?
-
 ?
collagen type II + H2O cathepsin L2 exhibits collagenase activity by cleaving at multiple sites within the alpha1 and alpha2 triple helix regions (Col domains) Fasciola hepatica ?
-
 ?
additional information cathepsin L2 readily cleaves substrates with Pro in the P2 position and peptide substrates mimicking the repeating Gly-Pro-Xaa motifs Fasciola hepatica ?
-
 ?
additional information the cathepsin L zymogen is autocatalytically activated and processed to mature enzyme by incubation for 2 h at 37°C in 0.1 M sodium citrate buffer (pH 5.0) containing 2 mM dithiothreitol and 2.5 mM EDTA Fasciola hepatica ?
-
 ?
tosyl-GPK-7-amido-4-methylcoumarin + H2O
-
 Fasciola hepatica ?
-
 ?
tosyl-GPR-7-amido-4-methylcoumarin + H2O
-
 Fasciola hepatica ?
-
 ?
Z-Gly-Pro-Gly-Gly-Pro-Ala + H2O
-
 Fasciola hepatica Z-Gly-Pro + Gly-Gly + L-Pro-L-Ala
-
 ?
Z-Gly-Pro-Leu-Gly-Pro + H2O
-
 Fasciola hepatica Z-Gly-Pro + L-Leu + Gly + L-Pro
-
 ?
Z-Leu-Arg-NHMe + H2O
-
 Fasciola hepatica ?
-
 ?
Z-Phe-Arg-NHMe + H2O
-
 Fasciola hepatica ?
-
 ?
Z-VVR-7-amido-4-methylcoumarin + H2O
-
 Fasciola hepatica ?
-
 ?

Subunits

Subunits Comment Organism
? x * 24500, mature enzyme, SDS-PAGE Fasciola hepatica
? x * 37000, proenzyme, SDS-PAGE Fasciola hepatica

Synonyms

Synonyms Comment Organism
CL2
-
 Fasciola hepatica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.014
-
 Z-VVR-7-amido-4-methylcoumarin in 100 mM sodium phosphate buffer (pH 6.0) containing 1 mM dithiothreitol and 1 mM EDTA, at 37°C Fasciola hepatica
0.07
-
 Boc-VLK-7-amido-4-methylcoumarin in 100 mM sodium phosphate buffer (pH 6.0) containing 1 mM dithiothreitol and 1 mM EDTA, at 37°C Fasciola hepatica
0.09
-
 Z-Phe-Arg-NHMe in 100 mM sodium phosphate buffer (pH 6.0) containing 1 mM dithiothreitol and 1 mM EDTA, at 37°C Fasciola hepatica
0.18
-
 tosyl-GPK-7-amido-4-methylcoumarin in 100 mM sodium phosphate buffer (pH 6.0) containing 1 mM dithiothreitol and 1 mM EDTA, at 37°C Fasciola hepatica
0.23
-
 Boc-VPR-7-amido-4-methylcoumarin in 100 mM sodium phosphate buffer (pH 6.0) containing 1 mM dithiothreitol and 1 mM EDTA, at 37°C Fasciola hepatica
0.26
-
 tosyl-GPR-7-amido-4-methylcoumarin in 100 mM sodium phosphate buffer (pH 6.0) containing 1 mM dithiothreitol and 1 mM EDTA, at 37°C Fasciola hepatica
0.55
-
 Boc-AGPR-7-amido-4-methylcoumarin in 100 mM sodium phosphate buffer (pH 6.0) containing 1 mM dithiothreitol and 1 mM EDTA, at 37°C Fasciola hepatica
0.95
-
 Z-Leu-Arg-NHMe in 100 mM sodium phosphate buffer (pH 6.0) containing 1 mM dithiothreitol and 1 mM EDTA, at 37°C Fasciola hepatica

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00000046
-
 cathepsin K inhibitor II in 100 mM sodium phosphate buffer (pH 6.0) containing 1 mM dithiothreitol and 1 mM EDTA, at 37°C Fasciola hepatica
0.00000532
-
 Z-Phe-Ala-CHN2 in 100 mM sodium phosphate buffer (pH 6.0) containing 1 mM dithiothreitol and 1 mM EDTA, at 37°C Fasciola hepatica

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
9.238
-
 Z-VVR-7-amido-4-methylcoumarin in 100 mM sodium phosphate buffer (pH 6.0) containing 1 mM dithiothreitol and 1 mM EDTA, at 37°C Fasciola hepatica
11.09
-
 Z-Phe-Arg-NHMe in 100 mM sodium phosphate buffer (pH 6.0) containing 1 mM dithiothreitol and 1 mM EDTA, at 37°C Fasciola hepatica
13.75
-
 tosyl-GPK-7-amido-4-methylcoumarin in 100 mM sodium phosphate buffer (pH 6.0) containing 1 mM dithiothreitol and 1 mM EDTA, at 37°C Fasciola hepatica
18.56
-
 tosyl-GPR-7-amido-4-methylcoumarin in 100 mM sodium phosphate buffer (pH 6.0) containing 1 mM dithiothreitol and 1 mM EDTA, at 37°C Fasciola hepatica
20.19
-
 Boc-VPR-7-amido-4-methylcoumarin in 100 mM sodium phosphate buffer (pH 6.0) containing 1 mM dithiothreitol and 1 mM EDTA, at 37°C Fasciola hepatica
30.71
-
 Boc-VLK-7-amido-4-methylcoumarin in 100 mM sodium phosphate buffer (pH 6.0) containing 1 mM dithiothreitol and 1 mM EDTA, at 37°C Fasciola hepatica
58.03
-
 Boc-AGPR-7-amido-4-methylcoumarin in 100 mM sodium phosphate buffer (pH 6.0) containing 1 mM dithiothreitol and 1 mM EDTA, at 37°C Fasciola hepatica
444.1
-
 Z-Leu-Arg-NHMe in 100 mM sodium phosphate buffer (pH 6.0) containing 1 mM dithiothreitol and 1 mM EDTA, at 37°C Fasciola hepatica