Literature summary for 3.4.22.B60 extracted from

Robinson, M.W.; Tort, J.F.; Lowther, J.; Donnelly, S.M.; Wong, E.; Xu, W.; Stack, C.M.; Padula, M.; Herbert, B.; Dalton, J.P.
Proteomics and phylogenetic analysis of the cathepsin L protease family of the helminth pathogen Fasciola hepatica: expansion of a repertoire of virulence-associated factors (2008), Mol. Cell. Proteomics, 7, 1111-1123.

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KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0036	-	benzyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin	37°C, pH 7.3	Fasciola hepatica
0.0044	-	benzyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin	37°C, pH 5.5	Fasciola hepatica
0.016	-	benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin	37°C, pH 7.3	Fasciola hepatica
0.0399	-	benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin	37°C, pH 5.5	Fasciola hepatica
0.0752	-	benzyloxycarbonyl-Pro-Arg-7-amido-4-methylcoumarin	37°C, pH 7.3	Fasciola hepatica
0.084	-	benzyloxycarbonyl-Pro-Arg-7-amido-4-methylcoumarin	37°C, pH 5.5	Fasciola hepatica

Organism

Organism	UniProt	Comment	Textmining
Fasciola hepatica	Q7JNQ8	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
benzyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin + H2O	-	Fasciola hepatica	benzyloxycarbonyl-Leu-Arg + 7-amino-4-methylcoumarin	-	?
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O	-	Fasciola hepatica	benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin	-	?
benzyloxycarbonyl-Pro-Arg-7-amido-4-methylcoumarin + H2O	-	Fasciola hepatica	benzyloxycarbonyl-Pro-Arg + 7-amino-4-methylcoumarin	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.4	-	benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin	37°C, pH 7.3	Fasciola hepatica
1.6	-	benzyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin	37°C, pH 5.5	Fasciola hepatica
1.7	-	benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin	37°C, pH 5.5	Fasciola hepatica
1.9	-	benzyloxycarbonyl-Pro-Arg-7-amido-4-methylcoumarin	37°C, pH 7.3	Fasciola hepatica
2.6	-	benzyloxycarbonyl-Pro-Arg-7-amido-4-methylcoumarin	37°C, pH 5.5	Fasciola hepatica
3.2	-	benzyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin	37°C, pH 7.3	Fasciola hepatica

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Release 2023.1 (January 2023)