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Literature summary for 3.4.22.B60 extracted from

  • Smooker, P.M.; Whisstock, J.C.; Irving, J.A.
    Siyaguna, S.; Spithill, T.W.: Pike, R.N.: A single amino acid substitution affects substrate specificity in cysteine proteinases from Fasciola hepatica (2000), Protein Sci., 9, 2567-2572.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.22.B60

Cloned(Commentary)

Cloned (Comment) Organism
expression in Saccharomyces cerevisiae Fasciola hepatica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0155
-
 benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin FhCatL2 Fasciola hepatica
0.0327
-
 tosyl-Gly-Phe-Arg-7-amido-4-methylcoumarin FhCatL2 Fasciola hepatica
0.0483
-
 tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin FhCatL2 Fasciola hepatica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
 x * 26000, mature enzyme, SDS-PAGE Fasciola hepatica

Organism

Organism UniProt Comment Textmining
Fasciola hepatica
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification 38000 Da proenzyme, 26000 Da mature enzyme Fasciola hepatica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O cathepsin L2 Fasciola hepatica benzyloxycarbonyl-Phe-Arg + 7-amino-4-methyl coumarin
-
 ?
tosyl-Gly-Phe-Arg-7-amido-4-methylcoumarin + H2O cathepsin L2 Fasciola hepatica tosyl-Gly-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin + H2O cathepsin L2 Fasciola hepatica tosyl-Gly-Pro-Arg + 7-amino-4-methyl coumarin
-
 ?

Subunits

Subunits Comment Organism
? x * 26000, mature enzyme, SDS-PAGE Fasciola hepatica

Synonyms

Synonyms Comment Organism
cathepsin L2
-
 Fasciola hepatica
FhCatL2
-
 Fasciola hepatica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
26.3
-
 tosyl-Gly-Phe-Arg-7-amido-4-methylcoumarin FhCatL2 Fasciola hepatica
50
-
 benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin FhCatL2 Fasciola hepatica
440
-
 tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin FhCatL2 Fasciola hepatica