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Literature summary for 3.4.22.B6 extracted from

  • Doleckova, K.; Kasny, M.; Mikes, L.; Cartwright, J.; Jedelsky, P.; Schneider, E.; Dvorak, J.; Mountford, A.; Craik, C.; Horak, P.
    The functional expression and characterisation of a cysteine peptidase from the invasive stage of the neuropathogenic schistosome Trichobilharzia regenti (2009), Int. J. Parasitol., 39, 201-211.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.22.B6

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris Trichobilharzia regenti

Inhibitors

Inhibitors Comment Organism Structure
N-(L-3-trans-propylcarbamoyloxirane-2-carbonyl)-Ile-Pro-OH CA-074, irreversible inhibitor Trichobilharzia regenti
N-(Na-carbonyl-Cpd-Gln-Ala-al)-Leu elastatinal, irreversible inhibitor Trichobilharzia regenti
N-trans-(epoxysuccinyl)-L-leucine 4-guanidinobutylamide E-64, irreversible inhibitor Trichobilharzia regenti
Z-Phe-Ala-diazomethylketone irreversible inhibitor Trichobilharzia regenti
Z-Phe-Phe-diazomethylketone irreversible inhibitor Trichobilharzia regenti
Z-Phe-Tyr(tBu)-diazomethylketone irreversible inhibitor Trichobilharzia regenti

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28500
-
 x * 28500, calculated for mature enzyme, x * 32000, SDS-PAGE (glycosylated) Trichobilharzia regenti
32000
-
 x * 28500, calculated for mature enzyme, x * 32000, SDS-PAGE (glycosylated) Trichobilharzia regenti

Organism

Organism UniProt Comment Textmining
Trichobilharzia regenti A7L844
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Trichobilharzia regenti

Purification (Commentary)

Purification (Comment) Organism
cation-exchange chromatography (Mono S) Trichobilharzia regenti

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Boc-Val-Leu-Lys-7-amido-4-methylcoumarin + H2O
-
 Trichobilharzia regenti Boc-Val-Leu-Lys + 7-amino-4-methylcoumarin
-
 ?
additional information collagen type IV, elastin, keratin, fibrinogen type III and myelin basic protein are degraded Trichobilharzia regenti ?
-
 ?
Z-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
 Trichobilharzia regenti Z-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
Z-Gly-Pro-Arg-7-amido-4-methylcoumarin + H2O
-
 Trichobilharzia regenti Z-Gly-Pro-Arg + 7-amino-4-methylcoumarin
-
 ?
Z-Phe-Arg-7-amido-4-methylcoumarin + H2O
-
 Trichobilharzia regenti Z-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?

Subunits

Subunits Comment Organism
? x * 28500, calculated for mature enzyme, x * 32000, SDS-PAGE (glycosylated) Trichobilharzia regenti

Synonyms

Synonyms Comment Organism
cathepsin B-like peptidase
-
 Trichobilharzia regenti
TrCB2
-
 Trichobilharzia regenti

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 substrate Z-Arg-Arg-7-amido-4-methylcoumarin Trichobilharzia regenti

pH Range

pH Minimum pH Maximum Comment Organism
4 6.5
-
 Trichobilharzia regenti

pI Value

Organism Comment pI Value Maximum pI Value
Trichobilharzia regenti calculated for mature enzyme
-
 5.9