Activating Compound | Comment | Organism | Structure |
---|---|---|---|
DTT | no activity in buffer lacking DTT | Trypanosoma congolense |
Application | Comment | Organism |
---|---|---|
medicine | TcoCBc1 elicits an important immune response in experimentally infected cattle. The protein is a potential therapeutic target and is a plausible antigen for Trypanosoma congolense diagnosis | Trypanosoma congolense |
Cloned (Comment) | Organism |
---|---|
six-His-tagged protein is expressed in Pichia pastoris strain X-33 | Trypanosoma congolense |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetyl-Leu-Val-lysinal | 350 nM, 37°C, pH 4.5, 1 h, 15% inhibition | Trypanosoma congolense | |
CA074 | 350 nM, 37°C, pH 4.5, 1 h, complete inhibition | Trypanosoma congolense | |
CBZ-Phe-Gly-NHO-Bz-pMe | 350 nM, 37°X, pH 4.5, 1 h, 87% inhibition | Trypanosoma congolense | |
cystatin | 350 nM, 37°C, pH 4.5, 1 h, 23% inhibition | Trypanosoma congolense | |
E-64 | 350 nM, 37°C, pH 4.5, 1 h, complete inhibition | Trypanosoma congolense |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1234 | - |
Nalpha-benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin | - |
Trypanosoma congolense | |
0.1733 | - |
N2-benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin | - |
Trypanosoma congolense |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
lysosome | - |
Trypanosoma congolense | 5764 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
SDS-PAGE analysis of the eluted recombinant protein reveals a prominent band of 39000 Da and a minor protein of 40000 Da. These bands correspond to glycosylated forms of a 35000 Da protein, the expected size of the protease | Trypanosoma congolense |
additional information | - |
SDS-PAGE analysis of the eluted recombinant protein reveals a prominent band of 40000 Da and a minor protein of 50000 Da. These bands correspond to glycosylated forms of a 36000 Da protein, the expected size of the protease | Trypanosoma congolense |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma congolense | - |
- |
- |
Trypanosoma congolense | B2C323 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | TcoCBc1 is able to autocatalyze its maturation 21 residues upstream of the predicted start of the catalytic domain | Trypanosoma congolense |
proteolytic modification | TcoCBc6 is able to autocatalyze its maturation 31 residues, upstream of the predicted start of the catalytic domain | Trypanosoma congolense |
Purification (Comment) | Organism |
---|---|
- |
Trypanosoma congolense |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
bloodstream form | - |
Trypanosoma congolense | - |
bloodstream form | expressed mainly in bloodstream form | Trypanosoma congolense | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-aminobenzoyl-Phe-Arg-Ala-(2,4-dinitrophenyl)-epsilon-NH2-lysine + H2O | - |
Trypanosoma congolense | ? | - |
? | |
benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O | - |
Trypanosoma congolense | CBZ-Arg-Arg + 7-amino-4-methylcoumarin | - |
? | |
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O | - |
Trypanosoma congolense | benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin | - |
? | |
additional information | no hydrolysis of benzoyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin and benzoyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin | Trypanosoma congolense | ? | - |
? | |
Nalpha-benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O | - |
Trypanosoma congolense | Nalpha-benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CBc1 | - |
Trypanosoma congolense |
CBc6 | - |
Trypanosoma congolense |
TcoCBc1 | - |
Trypanosoma congolense |
TcoCBc6 | - |
Trypanosoma congolense |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.09 | - |
Nalpha-benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin | - |
Trypanosoma congolense | |
3 | 6 | CBZ-Arg-Arg-7-amido-4-methylcoumarin | - |
Trypanosoma congolense | |
3.49 | - |
N2-benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin | - |
Trypanosoma congolense |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
- |
Trypanosoma congolense |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 6.5 | pH 4.0: about 30% of maximal activity, pH 6.5: about 75% of maximal activity | Trypanosoma congolense |