Literature summary for 3.4.22.B50 extracted from

Mielech, A.M.; Deng, X.; Chen, Y.; Kindler, E.; Wheeler, D.L.; Mesecar, A.D.; Thiel, V.; Perlman, S.; Baker, S.C.
Murine coronavirus ubiquitin-like domain is important for papain-like protease stability and viral pathogenesis (2015), J. Virol., 89, 4907-4917 .

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Organism

Organism	UniProt	Comment	Textmining
Murine coronavirus	-	-	-

Synonyms

Synonyms	Comment	Organism
PLP2	-	Murine coronavirus

General Information

General Information	Comment	Organism
physiological function	changes within the Ubl domain, residues 785 to 787 of nonstructural protein 3, negatively affect protease activity. Ubl mutant viruses V787S and V785S replicate efficiently at 37°C but generate smaller plaques than wild-type virus, and V787S is defective for replication at higher temperatures. The proteases of the mutant viruses exhibit similar specific activities at 25°C but diplay significantly reduced thermal stability at 30°C, thereby reducing the total enzymatic activity. Infection of C57BL/6 mice with V787S is highly attenuated, yet it replicates sufficiently to elicit protective immunity	Murine coronavirus

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Release 2023.1 (January 2023)