Literature summary for 3.4.22.B50 extracted from

Chu, H.F.; Chen, C.C.; Moses, D.C.; Chen, Y.H.; Lin, C.H.; Tsai, Y.C.; Chou, C.Y.
Porcine epidemic diarrhea virus papain-like protease 2 can be noncompetitively inhibited by 6-thioguanine (2018), Antiviral Res., 158, 199-205 .

Search for more literature for 3.4.22.B50

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	porcine epidemic diarrhea virus

Crystallization (Commentary)

Crystallization (Comment)	Organism
modeling of structure	porcine epidemic diarrhea virus

Protein Variants

Protein Variants	Comment	Organism
T39W	mutation enhances hydrolysis of the SARS-CoV-derived peptidyl substrate Dabcyl-FRLKGGAPIKGV-Edans	porcine epidemic diarrhea virus

Inhibitors

Inhibitors	Comment	Organism	Structure
6-Mercaptopurine	-	porcine epidemic diarrhea virus
6-thioguanine	noncompetitive	porcine epidemic diarrhea virus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0186	-	Dabcyl-FRLKGGAPIKGV-Edans	wild-type, pH 6.5, 30°C	porcine epidemic diarrhea virus
0.0321	-	Dabcyl-FRLKGGAPIKGV-Edans	mutant T39W, pH 6.5, 30°C	porcine epidemic diarrhea virus
0.0396	-	Dabcyl-FKKKGGGDVKE-Edans	mutant T39W, pH 6.5, 30°C	porcine epidemic diarrhea virus
0.0616	-	Dabcyl-FKKKGGGDVKE-Edans	wild-type, pH 6.5, 30°C	porcine epidemic diarrhea virus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
25000	-	gel filtration	porcine epidemic diarrhea virus

Organism

Organism	UniProt	Comment	Textmining
porcine epidemic diarrhea virus	P0C6Y4	polyprotein	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Dabcyl-FKKKGGGDVKE-Edans + H2O	-	porcine epidemic diarrhea virus	?	-	?
Dabcyl-FRLKGGAPIKGV-Edans + H2O	SARS-CoV-derived substrate	porcine epidemic diarrhea virus	?	-	?
ubiquitin-7-amido-4-trifluoro-methyl-coumarin + H2O	-	porcine epidemic diarrhea virus	ubiquitin + 7-amino-4-trifluoro-methyl-coumarin	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 25800, calculated from sequence	porcine epidemic diarrhea virus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.0011	-	Dabcyl-FRLKGGAPIKGV-Edans	wild-type, pH 6.5, 30°C	porcine epidemic diarrhea virus
0.0041	-	Dabcyl-FKKKGGGDVKE-Edans	mutant T39W, pH 6.5, 30°C	porcine epidemic diarrhea virus
0.005	-	Dabcyl-FKKKGGGDVKE-Edans	wild-type, pH 6.5, 30°C	porcine epidemic diarrhea virus
0.0175	-	Dabcyl-FRLKGGAPIKGV-Edans	mutant T39W, pH 6.5, 30°C	porcine epidemic diarrhea virus

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.0211	-	6-thioguanine	pH 6.5, 30°C	porcine epidemic diarrhea virus

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.0137	-	pH 6.5, 30°C	porcine epidemic diarrhea virus	6-thioguanine
0.0581	-	pH 6.5, 30°C	porcine epidemic diarrhea virus	6-Mercaptopurine

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.055	-	Dabcyl-FRLKGGAPIKGV-Edans	mutant T39W, pH 6.5, 30°C	porcine epidemic diarrhea virus
0.058	-	Dabcyl-FRLKGGAPIKGV-Edans	wild-type, pH 6.5, 30°C	porcine epidemic diarrhea virus
0.082	-	Dabcyl-FKKKGGGDVKE-Edans	wild-type, pH 6.5, 30°C	porcine epidemic diarrhea virus
0.103	-	Dabcyl-FKKKGGGDVKE-Edans	mutant T39W, pH 6.5, 30°C	porcine epidemic diarrhea virus

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Release 2023.1 (January 2023)