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Literature summary for 3.4.22.B50 extracted from

  • Kong, L.; Shaw, N.; Yan, L.; Lou, Z.; Rao, Z.
    Structural view and substrate specificity of papain-like protease from avian infectious bronchitis virus (2015), J. Biol. Chem., 290, 7160-7168.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.22.B50

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells infectious bronchitis virus

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 100 mM Bis-Tris, pH 6.5, 25-30% (w/v) polyethylene glycol monomethyl ether 2000 infectious bronchitis virus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.67
-
 Z-LRGG-7-amido-4-methylcoumarin in 20 mM Tris-HCl, pH 7.5, 0.1 mg/ml BSA, 150 mM NaCl, 2 mM dithiothreitol, at 25°C infectious bronchitis virus
3.23
-
 Z-KKAG-7-amido-4-methylcoumarin in 20 mM Tris-HCl, pH 7.5, 0.1 mg/ml BSA, 150 mM NaCl, 2 mM dithiothreitol, at 25°C infectious bronchitis virus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
polyubiquitin + H2O infectious bronchitis virus the enzyme cleaves Lys48- and Lys63-linked polyubiquitin to monoubiquitin but not linear polyubiquitin monoubiquitin + ?
-
 ?

Organism

Organism UniProt Comment Textmining
infectious bronchitis virus P0C6Y1
-
-

Purification (Commentary)

Purification (Comment) Organism
GST resin column chromatography, Mono Q column chromatography, and Superdex 75 gel filtration infectious bronchitis virus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme cannot cleave Z-KAGG-7-amido-4-methylcoumarin infectious bronchitis virus ?
-
 ?
polyubiquitin + H2O the enzyme cleaves Lys48- and Lys63-linked polyubiquitin to monoubiquitin but not linear polyubiquitin infectious bronchitis virus monoubiquitin + ?
-
 ?
Z-KKAG-7-amido-4-methylcoumarin + H2O the catalytic efficiency toward Z-KKAG-7-amido-4-methylcoumarin is 5times higher than that for Z-LRGG-7-amido-4-methylcoumarin infectious bronchitis virus Z-KKAG + 7-amino-4-methylcoumarin
-
 ?
Z-LRGG-7-amido-4-methylcoumarin + H2O
-
 infectious bronchitis virus Z-LRGG + 7-amino-4-methylcoumarin
-
 ?

Synonyms

Synonyms Comment Organism
PLpro
-
 infectious bronchitis virus