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Literature summary for 3.4.22.B5 extracted from

  • Elias, C.G.; Pereira, F.M.; Dias, F.A.; Silva, T.L.; Lopes, A.H.; dAvila-Levy, C.M.; Branquinha, M.H.; Santos, A.L.
    Cysteine peptidases in the tomato trypanosomatid Phytomonas serpens: influence of growth conditions, similarities with cruzipain and secretion to the extracellular environment (2008), Exp. Parasitol., 120, 343-352.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol 2 milliM, pH 5.0, 48 h, 37°C, enzymatic activity depends on reducing agents Phytomonas serpens
L-cysteine 2 milliM, pH 5.0, 48 h, 37°C, enzymatic activity depends on reducing agents Phytomonas serpens

Inhibitors

Inhibitors Comment Organism Structure
cystatin 1 microM, 37°C, 48 h, pH 5.0 Phytomonas serpens
leupeptin 1 microM, 37°C, 48 h, pH 5.0 Phytomonas serpens
trans-epoxysuccinyl-L-leucylamido(4-guanidino)butane 1 microM, 37°C, 48 h, pH 5.0 Phytomonas serpens

Localization

Localization Comment Organism GeneOntology No. Textmining
cell surface attached to membrane domains via glycosylphophatidylinositol anchor Phytomonas serpens 9986
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
Western blotting Phytomonas serpens
40000
-
Western blotting Phytomonas serpens

Organism

Organism UniProt Comment Textmining
Phytomonas serpens
-
isolate 9T
-

Storage Stability

Storage Stability Organism
37°C, 48 h, pH 5.0 Phytomonas serpens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
albumin 37°C, 48 h, pH 5.0 Phytomonas serpens ?
-
?
casein 37°C, 48 h, pH 5.0 Phytomonas serpens ?
-
?
gelatin 37°C, 48 h, pH 5.0 Phytomonas serpens ?
-
?
haemoglobin 37°C, 48 h, pH 5.0 Phytomonas serpens ?
-
?
mucin 37°C, 48 h, pH 5.0 Phytomonas serpens ?
-
?
non-immune immunoglobulin 37°C, 48 h, pH 5.0 Phytomonas serpens ?
-
?
salivary gland extract 37°C, 48 h, pH 5.0, salivary gland extract from Oncopeltus fasciatus Phytomonas serpens ?
-
?

Synonyms

Synonyms Comment Organism
cysteine peptidase
-
Phytomonas serpens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
pH 5.0, 48 h Phytomonas serpens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
37°C Phytomonas serpens

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 9 37°C, decreased enzymatic activity at pH 3.0 or neutral-alkaline pH values, 48 h Phytomonas serpens