Literature summary for 3.4.22.B49 extracted from

Sansri, V.; Meemon, K.; Changklungmoa, N.; Kueakhai, P.; Chantree, P.; Chaichanasak, P.; Lorsuwannarat, N.; Itagaki, T.; Sobhon, P.
Protection against Fasciola gigantica infection in mice by vaccination with recombinant juvenile-specific cathepsin L (2015), Vaccine, 33, 1596-1601 .

Search for more literature for 3.4.22.B49

Application

Application	Comment	Organism
diagnostics	recombinant proFgCatL1H protein expressed from Pichia pastoris is mixed with Freund's adjuvants and used to subcutaneously immunize mice, the mice are then challenged with metacercariae of Fasciola gigantica. The percentage of worm protection in the rproFgCatL1H-vaccinated mice compared to the non-immunized and adjuvant control mice are approximately 62.7% and 66.1%, respectively. Anti-rproFgCatL1H antisera collected from vaccinated mice react specifically with rproFgCatL1H and other cathepsin L isoforms of Fasciola gigantica, but the antibodies do not crossreact with antigens from other trematode and nematode parasites, including Eurytrema pancreaticum, Opisthorchis viverrini, Fischoederius cobboldi, Cotylophoron cotylophorum, Gigantocotyle explanatum, Paramphistomum cervi, and Setarialabiato papillosa	Fasciola gigantica
medicine	recombinant proFgCatL1H protein expressed from Pichia pastoris is mixed with Freund's adjuvants and used to subcutaneously immunize mice, the mice are then challenged with metacercariae of Fasciola gigantica. The percentage of worm protection in the rproFgCatL1H-vaccinated mice compared to the non-immunized and adjuvant control mice are approximately 62.7% and 66.1%, respectively. The levels of IgG1 and IgG2a in the immune sera are shown to be strongly correlated with the numbers of worm recovery, and the correlation coefficient is higher for IgG1. The levels of serum aspartate amino-transferase and alanine transaminase are significantly lower in the sera of rproFgCatL1H-vaccinated mice than in the infected control mice indicating a lower degree of liver damage	Fasciola gigantica

Cloned(Commentary)

Cloned (Comment)	Organism
recombinant expression of proFgCatL1H in Pichia pastoris strain GS115	Fasciola gigantica

Organism

Organism	UniProt	Comment	Textmining
Fasciola gigantica	Q6T857	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant proFgCatL1H from Pichia pastoris strain GS115	Fasciola gigantica

Synonyms

Synonyms	Comment	Organism
cat-L1H	-	Fasciola gigantica
cathepsin L1H	-	Fasciola gigantica
FgCatL1H	-	Fasciola gigantica

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Release 2023.1 (January 2023)