Application | Comment | Organism |
---|---|---|
diagnostics | analysis of the diagnostic values of the three different clades of cathepsin Ls, FhCL1, FhCL2, and FhCL5, from adult flukes in an ELISA, test of sera from sheep and cattle naturally infected with Fasciola hepatica, of cross-reactive antibodies, overview. For sheep sera, the sensitivity is 100% for the three rFhpCLs, while for cattle sera, the highest sensitivity is obtained using rFhpCL2 (97%), being equal for both rFhpCL1 and rFhpCL5 (87.9%), after adjusting cut-offs for maximum specificity | Fasciola hepatica |
Cloned (Comment) | Organism |
---|---|
gene FhpCL1, DNA and amino acid sequence determination and analysis, sequence comparisons of rFhpCLs, recombinant expression of His-tagged enzyme in Escherichia coli | Fasciola hepatica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Fasciola hepatica | H8WG11 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant solubilized and refolded His-tagged enzyme from Escherichia coli by nickel affinity chromatography | Fasciola hepatica |
Renatured (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli inclusion bodies, solubilization by 8 M urea, followed by dilution, dialysis, and ultrafiltration | Fasciola hepatica |
Synonyms | Comment | Organism |
---|---|---|
FhpCL1 | - |
Fasciola hepatica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Fasciola hepatica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Fasciola hepatica |
General Information | Comment | Organism |
---|---|---|
evolution | adult flukes produce three different clades of cathepsin Ls: FhCL1, FhCL2, and FhCL5 | Fasciola hepatica |