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Literature summary for 3.4.22.B49 extracted from
- Molecular characterization of a cathepsin L1 highly expressed in phagocytes of pacific oyster Crassostrea gigas (2018), Dev. Comp. Immunol., 89, 152-162 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene CGI_10027418, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, functional recombinant expression of His-tagged enzyme in Escherichia coli strain Rosetta (DE3) | Crassostrea gigas |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Crassostrea gigas | 5737 | - |
| lysosome | - |
Crassostrea gigas | 5764 | - |
| additional information | the subcellular localization of CgCTSL1 protein in hemocyte is detected by immunofluorescence | Crassostrea gigas | - |
- |
| nucleus | - |
Crassostrea gigas | 5634 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Crassostrea gigas | K1QEW8 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain Rosetta (DE3) by nickel affinity chromatography and two steps of dialysis | Crassostrea gigas |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| adductor | low enzyme content | Crassostrea gigas | - |
| gill | moderate enzyme content | Crassostrea gigas | - |
| gonad | low enzyme content | Crassostrea gigas | - |
| hemocyte | high enzyme content, proteolytic activity and distribution of CgCTSL1 in different subpopulations of hemocytes, distribution of CgCTSL1 in phagocytes and non-phagocytic hemocytes, overview | Crassostrea gigas | - |
| hepatopancreas | - |
Crassostrea gigas | - |
| mantle | moderate enzyme content | Crassostrea gigas | - |
| phagocyte | phagocytic hemocytes, cathepsin L1 is highly expressed in phagocytes | Crassostrea gigas | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-FR-7-amido-4-trifluoromethyl coumarin + H2O | - |
Crassostrea gigas | acetyl-FR + 7-amino-4-trifluoromethyl coumarin | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 23030, sequence calculation | Crassostrea gigas |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CgCTSL1 | - |
Crassostrea gigas |
| CGI_10027418 | - |
Crassostrea gigas |
| cysteine proteinase 3 | - |
Crassostrea gigas |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Crassostrea gigas |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Crassostrea gigas |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Crassostrea gigas | sequence calculation | - |
8.07 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | cathepsin L1 is a lysosomal cysteine protease with a papain-like structure. The CTSL1 homologue CgCTSL1 is identified from Crassostrea gigas as a protein containing a typical single Pept_C1 domain with three conserved catalytically essential residues (Gln25, His135 and Asn178) | Crassostrea gigas |
| physiological function | cathepsin L1 (CTSL1) is implicated in multiple processes of immune response against pathogen infection based on the proteolytic activity | Crassostrea gigas |
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