Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.22.B49 extracted from

  • Ben-Mahmoud, S.; Ramos, J.E.; Shatters, R.G.; Rouge, P.; Powell, C.A.; Smagghe, G.; Borovsky, D.
    Cloning and characterization of a basic cysteine-like protease (cathepsin L1) expressed in the gut of larval Diaprepes abbreviatus L. (Coleoptera: Curculionidae) (2015), J. Insect Physiol., 72, 1-13.
    View publication on PubMed
Search for more literature for 3.4.22.B49

Cloned(Commentary)

Cloned (Comment) Organism
expressed as a fusion protein with GST and His6 tags in Escherichia coli Rosetta (DE3) pLysS cells Diaprepes abbreviatus
expressed in Escherichia coli Rosetta (DE3) pLysS cells Diaprepes abbreviatus

Inhibitors

Inhibitors Comment Organism Structure
Aprotinin complete inhibition at 50 nM at a molar ratio of enzyme to inhibitor of 17:1; inhibits enzyme activity almost completely at a molar ratio of enzyme to inhibitor of 17:1 Diaprepes abbreviatus
CA074 25.3% inhibition at 0.05 mM Diaprepes abbreviatus
chymostatin 85.6% inhibition at 0.05 mM Diaprepes abbreviatus
cystatin 47.5% inhibition at 0.05 mM Diaprepes abbreviatus
E-64 i.e. L-trans-epoxysuccinyl-leucyamido(4-guanidino) butane, 26.6% inhibition at 0.05 mM Diaprepes abbreviatus
N-benzyloxycarbonylphenylalanyl-t-butyl-tryrosyl diazomethylketone 40.6% inhibition at 0.05 mM Diaprepes abbreviatus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23000
-
 x * 23000, active enzyme, SDS-PAGE Diaprepes abbreviatus
23000
-
 x * 23000, mature enzyme, SDS-PAGE Diaprepes abbreviatus
60000
-
 x * 60000, mature GST- and His6-tagged enzyme, SDS-PAGE Diaprepes abbreviatus
60000
-
 x * 60000, recombinant enzyme with GST and His6 tags, SDS-PAGE Diaprepes abbreviatus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Gelatin + H2O Diaprepes abbreviatus
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Diaprepes abbreviatus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
in the presence of 1% Triton X-100 by Ni-NTA affinity column chromatography and Sephacryl S-100 gel filtration Diaprepes abbreviatus
Ni-NTA affinity column chromatography and gel filtration Diaprepes abbreviatus

Source Tissue

Source Tissue Comment Organism Textmining
gut
-
 Diaprepes abbreviatus
-
larva
-
 Diaprepes abbreviatus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyloxycarbonyl-Phe-Arg-4-nitroanilide + H2O
-
 Diaprepes abbreviatus benzyloxycarbonyl-Phe-Arg + 4-nitroaniline
-
 ?
benzyloxycarbonyl-Phe-Arg-7-amido-4-trifluoromethylcoumarin + H2O best substrate Diaprepes abbreviatus benzyloxycarbonyl-Phe-Arg + 7-amino-4-trifluoromethylcoumarin
-
 ?
Gelatin + H2O
-
 Diaprepes abbreviatus ?
-
 ?
additional information benzyloxycarbonyl-Gly-Pro-Atg-7-amido-4-methylcoumarin and benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin are not hydrolyzed Diaprepes abbreviatus ?
-
 ?
additional information does not hydrolyze Arg-4-nitroanilide, N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide, benzyloxycarbonyl-Gly-Pro-Arg-7-amido-4-methylcoumarin, and benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin Diaprepes abbreviatus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 23000, active enzyme, SDS-PAGE Diaprepes abbreviatus
? x * 23000, mature enzyme, SDS-PAGE Diaprepes abbreviatus
? x * 60000, mature GST- and His6-tagged enzyme, SDS-PAGE Diaprepes abbreviatus
? x * 60000, recombinant enzyme with GST and His6 tags, SDS-PAGE Diaprepes abbreviatus

Synonyms

Synonyms Comment Organism
cathepsin L1
-
 Diaprepes abbreviatus
CTSL1
-
 Diaprepes abbreviatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Diaprepes abbreviatus