Literature summary for 3.4.22.B47 extracted from

Kembhavi, A.A.; Buttle, D.J.; Knight, C.G.; Barrett, A.J.
The two cysteine endopeptidases of legume seeds: purification and characterization by use of specific fluorometric assays (1993), Arch. Biochem. Biophys., 303, 208-213.

Search for more literature for 3.4.22.B47

Inhibitors

Inhibitors	Comment	Organism	Structure
cystatin	reversible inhibition	Vigna aconitifolia
E-64	-	Vigna aconitifolia
iodoacetamide	exceptionally low rat constant for inhibition	Vigna aconitifolia
iodoacetate	exceptionally low rat constant for inhibition	Vigna aconitifolia
leupeptin	reversible inhibition	Vigna aconitifolia

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
27000	-	x * 27000, SDS-PAGE	Vigna aconitifolia

Organism

Organism	UniProt	Comment	Textmining
Vigna aconitifolia	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
additional information	the enzyme is not a glycoprotein	Vigna aconitifolia

Purification (Commentary)

Purification (Comment)	Organism
-	Vigna aconitifolia

Source Tissue

Source Tissue	Comment	Organism	Textmining
seed	activity increases during germination	Vigna aconitifolia	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
890	-	-	Vigna aconitifolia

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
azocasein + H2O	-	Vigna aconitifolia	?	-	?
benzoyl-Phe-Val-Arg-7-amido-4-methylcoumarin + H2O	4.4% of the activity with benzyloxycarbonyl-Phe-Arg-7-(4-methyl)coumarylamide	Vigna aconitifolia	benzoyl-Phe-Val-Arg + 7-amino-4-methylcoumarin	-	?
benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O	10% of the activity with benzyloxycarbonyl-Phe-Arg-7-(4-methyl)coumarylamide	Vigna aconitifolia	benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin	-	?
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O	-	Vigna aconitifolia	benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin	-	?
Gelatin + H2O	-	Vigna aconitifolia	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 27000, SDS-PAGE	Vigna aconitifolia

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
26.5	-	very stable during incubation in the range pH 3.0-7.0	Vigna aconitifolia

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5.5	-	reaction with azocasein	Vigna aconitifolia
7.5	-	activity with benzyloxycarbonyl-Phe-Arg-7-(4-methyl)coumarylamide	Vigna aconitifolia

pH Range

pH Minimum	pH Maximum	Comment	Organism
3.5	7.5	pH 3.5: about 40% of maximal activity, pH 7.5: about 65% of maximal activity, reaction with casein	Vigna aconitifolia
4.5	8	pH 4.5: about 35% of maximal activity, pH 8.0: about 60% of maximal activity, reaction with benzyloxycarbonyl-Phe-Arg-7-(4-methyl)coumarylamide	Vigna aconitifolia

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
3	7	stable at 26.5°C	Vigna aconitifolia

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.0000075	-	leupeptin	26°C, pH 6.0	Vigna aconitifolia
0.00003	-	cystatin	26°C, pH 6.0	Vigna aconitifolia

pI Value

Organism	Comment	pI Value Maximum	pI Value
Vigna aconitifolia	-	4.7	4.6

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Release 2023.1 (January 2023)