Cloned (Comment) | Organism |
---|---|
cloning and inducible expression of C-terminally V5/His-tagged CalpA in S2 cells | Drosophila melanogaster |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NF-kappa-B inhibitor cactus | negatively regulates calpain A activity. In Drosophila cactus exists as free and NFkappaB-bound forms | Drosophila melanogaster |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Drosophila melanogaster | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | dependent on | Drosophila melanogaster |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cactus + H2O | Drosophila melanogaster | an IkappaB homologue, Cactus and calpain A physically interact, calpain A generates a C-terminaltruncated Cactus fragment devoid of Toll-responsive sequences, cleavage analysis in vivo, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | Q11002 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | cactus regulates calpain A localization, calpain A redistribution to the cytoplasm requires full-length cactus, overview | Drosophila melanogaster | - |
oocyte | expression during early embryogenesis | Drosophila melanogaster | - |
syncytium | CalpA protein is apically enriched in the submembranous cytoplasm of the embryo syncytium | Drosophila melanogaster | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cactus + H2O | an IkappaB homologue, Cactus and calpain A physically interact, calpain A generates a C-terminaltruncated Cactus fragment devoid of Toll-responsive sequences, cleavage analysis in vivo, overview | Drosophila melanogaster | ? | - |
? | |
cactus + H2O | an IkappaB homologue, GFP-tagged substrate, calpain A generates a C-terminaltruncated Cactus fragment devoid of Toll-responsive sequences | Drosophila melanogaster | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CalpA | - |
Drosophila melanogaster |
calpain A | - |
Drosophila melanogaster |
General Information | Comment | Organism |
---|---|---|
additional information | cactus protein levels affect the cellular localization of the enzyme, overview. Wild-type syncytial blastoderm embryo show endogenous calpain A beneath the plasma membrane, while in loss-of-function cact mutants calpain A is diffuse in the cytoplasm. In a gain-of-function cact mutant, calpain A distribution is patchy during interphase and remains so during mitosis. Cactus regulates calpain A activity and localization | Drosophila melanogaster |
physiological function | calpain A regulates regulate NFkappaB function by different mechanisms, it decreases IkappaB homologue cactus levels in vivo and regulates cactus function in the immune system. The enzyme interacts physically with cactus, recognizing a Cactus pool that is not bound to Dorsal, a fly NFkappaB/Rel homologue. The enzyme targets free cactus, which is incorporated into and modulates Toll-responsive complexes in the embryo and immune system. Effect of calpain A on the embryonic DV axis. Cactus regulates calpain A activity and localization | Drosophila melanogaster |