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Literature summary for 3.4.22.B27 extracted from
- Involvement of calpain-7 in epidermal growth factor receptor degradation via the endosomal sorting pathway (2014), FEBS J., 281, 3642-3655.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant expression of wild-type enzyme and monomeric GFP-tagged protease-inactive CAPN7 mutant C290S in HeLa cells. The monomeric GFP-tagged inactive CAPN7 mutant C290S is mobilized to EGFR-positive endosomes upon epidermal growth factor stimulation in HeLa cells | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C290S | site-directed mutagenesis, catalytically inactive mutant, the mGFP-CAPN7C290S mutant exhibits dominant-negative effects on epidermal growth factor receptor degradation | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| endosome membrane | the enzyme functions on the endosomal membranes. Involvement of IST1 in mobilization of the enzyme to epidermal growth factor receptor-positive endosomes | Homo sapiens | 10008 | - |
| intracellular | - |
Homo sapiens | 5622 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | dependent on | Homo sapiens |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Homo sapiens | the enzyme interacts with a subset of endosomal sorting complex required for transport (ESCRT)-III and ESCRT-III-related proteins, including charged multivesicular body protein 1 | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | Q9Y6W3 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| HeLa cell | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme interacts with a subset of endosomal sorting complex required for transport (ESCRT)-III and ESCRT-III-related proteins, including charged multivesicular body protein 1 | Homo sapiens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | the enzyme possesses two MIT domains (MITa and MITb) at its N-terminus, a catalytic cysteine protease domain that is composed of PC-1 and PC-2 in the middle, and two CBSW, calpain-type beta-sandwich domains (CBSW) at its C-terminus | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| calpain-7 | - |
Homo sapiens |
| CAPN7 | - |
Homo sapiens |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | epidermal growth factor receptor degradation is slightly delayed in CAPN7-knockdown HeLa cells, the effect can be reversed by re-expression of wild-type enzyme CAPN7 but not a protease-inactive mutant | Homo sapiens |
| additional information | calpain-7 is a unique intracellular cysteine protease that has a tandem repeat of microtubule interacting and trafficking domains and lacks a penta-EF-hand domain. The catalytic residues are C290, H458 and N478 | Homo sapiens |
| physiological function | the enzyme is involved in membrane trafficking. The proteolytic activity of CAPN7 is important for the acceleration of epidermal growth factor receptor degradation via the endosomal sorting pathway utilizing a part of the endosomal sorting complex required for transport, ESCRT, system | Homo sapiens |
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