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Literature summary for 3.4.22.8 extracted from
- Purification and properties of clostridiopeptidase B (Clostripain) (1968), J. Biol. Chem., 243, 4683-4692.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | 0.01 mM, 30% inhibition | Hathewaya histolytica |  |
| Co2+ | 0.01 mM, 50% inhibition | Hathewaya histolytica | |
| Cu2+ | 0.01 mM, 30% inhibition | Hathewaya histolytica | |
| EDTA | - |
Hathewaya histolytica | |
| H2O2 | - |
Hathewaya histolytica | |
| K+ | 100 mM, 40% inhibition | Hathewaya histolytica | |
| Na+ | 100 mM, 40% inhibition | Hathewaya histolytica | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 1 mM, 50% enhancement | Hathewaya histolytica | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
equilibrium sedimentation | Hathewaya histolytica |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Hathewaya histolytica | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Hathewaya histolytica |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 66 | - |
- |
Hathewaya histolytica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | specificity is confined primarily to Arg residues | Hathewaya histolytica | ? | - |
? | |
| N-benzoyl-Arg amide + H2O | - |
Hathewaya histolytica | N-benzoyl-Arg + NH3 | - |
? | |
| N-benzoyl-Arg ethyl ester + H2O | - |
Hathewaya histolytica | N-benzoyl-Arg + ethanol | - |
? | |
| p-tosyl-Arg methyl ester + H2O | N-alpha-p-tosyl-L-Arg methyl ester | Hathewaya histolytica | p-tosyl-Arg + methanol | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | 7.8 | hydrolysis of alpha-benzoyl-Arg ethyl ester | Hathewaya histolytica |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 8.5 | about 35% of maximal activity at pH 6.5 and at pH 8.5, hydrolysis of benzoyl-Arg ethyl ester | Hathewaya histolytica |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
Co2+ | - |
Hathewaya histolytica | |
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