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Literature summary for 3.4.22.8 extracted from
- alpha-Clostripain. Chemical characterization, activity, and thiol content of the highly active form of clostripain (1979), J. Biol. Chem., 254, 1462-1468.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5,5'-dithiobis(2-nitrobenzoate) | - |
Hathewaya histolytica |  |
| diisopropylfluorophosphate | - |
Hathewaya histolytica | |
| iodoacetate | - |
Hathewaya histolytica | |
| macroglobulin | alpha2-macroglobulin from human plasma, inhibits both the amidase and protease activity. Macroglobulin from rat and dog plasma | Hathewaya histolytica | |
| N-alpha-p-tosyl-L-Lys chloromethyl ketone | - |
Hathewaya histolytica | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.26 | - |
alpha-benzoyl-Arg ethyl ester | - |
Hathewaya histolytica | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 12500 | - |
x * 12500, x * 43000, SDS-PAGE with or without 2-mercaptoethanol | Hathewaya histolytica |
| 38000 | - |
gel filtration | Hathewaya histolytica |
| 43000 | - |
x * 12500, x * 43000, SDS-PAGE with or without 2-mercaptoethanol | Hathewaya histolytica |
| 50000 | - |
analytical ultracentrifugation | Hathewaya histolytica |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Hathewaya histolytica | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 625.5 | - |
- |
Hathewaya histolytica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | cleaves proteins and synthetic substrates preferentially at the carboxyl group of Arg | Hathewaya histolytica | ? | - |
? | |
| N-benzoyl-Arg ethyl ester + H2O | - |
Hathewaya histolytica | N-benzoyl-Arg + ethanol | - |
? | |
| parvalbumin + H2O | exclusive cleavage of the Arg75-Ala76 bond | Hathewaya histolytica | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 12500, x * 43000, SDS-PAGE with or without 2-mercaptoethanol | Hathewaya histolytica |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 580 | - |
alpha-benzoyl-Arg ethyl ester | - |
Hathewaya histolytica | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 7.2 | hydrolysis of alpha-N-benzoyl-Arg ethyl ester | Hathewaya histolytica |
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