Literature summary for 3.4.22.8 extracted from

  • Mitchell, W.M.; Harrington, W.F.
    Clostripain (1971), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 3, 699-719.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
Borate partial inhibition Hathewaya histolytica
butylguanidine most effective competitive inhibitor, hydrolysis of benzoyl-Arg ethyl ester Hathewaya histolytica
Cd2+
-
Hathewaya histolytica
citrate partial inhibition Hathewaya histolytica
Co2+
-
Hathewaya histolytica
Cu2+
-
Hathewaya histolytica
EDTA
-
Hathewaya histolytica
H2O2 complete regeneration by reduction with dithiothreitol Hathewaya histolytica
K+
-
Hathewaya histolytica
Na+
-
Hathewaya histolytica
PCMB
-
Hathewaya histolytica
Polyarginine hydrolysis of benzoyl-Arg ethyl ester Hathewaya histolytica
polylysine hydrolysis of benzoyl-Arg ethyl ester Hathewaya histolytica
RCM RNase hydrolysis of benzoyl-Arg ethyl ester Hathewaya histolytica
Tris partial inhibition Hathewaya histolytica
Veronal partial inhibition Hathewaya histolytica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3
-
N-benzoyl-L-Arg amide
-
Hathewaya histolytica
0.022
-
p-toluenesulfonyl-L-Arg methyl ester
-
Hathewaya histolytica
0.25
-
Thr-Ala-Ala
-
Hathewaya histolytica
0.22
-
benzoyl-Arg ethyl ester
-
Hathewaya histolytica
2.5
-
p-Nitrophenyl p-guanidinobenzoate
-
Hathewaya histolytica

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ enhances esterase activity Hathewaya histolytica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
-
Hathewaya histolytica

Organism

Organism UniProt Comment Textmining
Hathewaya histolytica
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Hathewaya histolytica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the major activity is directed towards the carboxyl peptide linkage of Arg Hathewaya histolytica ?
-
?
Nalpha-benzoyl-L-Arg 2-naphthylamide + H2O
-
Hathewaya histolytica Nalpha-benzoyl-L-Arg + 2-naphthylamine
-
?
L-Arg methyl ester + H2O
-
Hathewaya histolytica L-Arg + methanol
-
?
N-benzoyl-Arg ethyl ester + H2O
-
Hathewaya histolytica N-benzoyl-Arg + ethanol
-
?
N-benzoyl-Arg amide + H2O
-
Hathewaya histolytica N-benzoyl-Arg + NH3
-
?
N-benzoyl-Arg methyl ester + H2O
-
Hathewaya histolytica N-benzoyl-Arg + methanol
-
?
N-benzoyl-Arg benzyl ester + H2O
-
Hathewaya histolytica N-benzoyl-Arg + benzyl alcohol
-
?
N-benzoyl-Arg isopropyl ester + H2O
-
Hathewaya histolytica N-benzoyl-Arg isopropanol
-
?
p-tosyl-Arg methyl ester + H2O
-
Hathewaya histolytica p-tosyl-Arg + methanol
-
?
p-nitrophenyl p-guanidinobenzoate + H2O
-
Hathewaya histolytica p-nitrophenol + p-guanidinobenzoate
-
?
Thr-Ala-Ala + H2O
-
Hathewaya histolytica ?
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
Ca2+ depresses thermal inactivation Hathewaya histolytica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
hydrolysis of L-Arg methyl ester, in phosphate buffer Hathewaya histolytica
7.4 7.8 hydrolysis of alpha-benzoyl-Arg ethyl ester Hathewaya histolytica
7.4 7.8 in phosphate or Tris-HCl buffer Hathewaya histolytica