Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Borate | partial inhibition | Hathewaya histolytica | |
butylguanidine | most effective competitive inhibitor, hydrolysis of benzoyl-Arg ethyl ester | Hathewaya histolytica | |
Cd2+ | - |
Hathewaya histolytica | |
citrate | partial inhibition | Hathewaya histolytica | |
Co2+ | - |
Hathewaya histolytica | |
Cu2+ | - |
Hathewaya histolytica | |
EDTA | - |
Hathewaya histolytica | |
H2O2 | complete regeneration by reduction with dithiothreitol | Hathewaya histolytica | |
K+ | - |
Hathewaya histolytica | |
Na+ | - |
Hathewaya histolytica | |
PCMB | - |
Hathewaya histolytica | |
Polyarginine | hydrolysis of benzoyl-Arg ethyl ester | Hathewaya histolytica | |
polylysine | hydrolysis of benzoyl-Arg ethyl ester | Hathewaya histolytica | |
RCM RNase | hydrolysis of benzoyl-Arg ethyl ester | Hathewaya histolytica | |
Tris | partial inhibition | Hathewaya histolytica | |
Veronal | partial inhibition | Hathewaya histolytica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.022 | - |
p-toluenesulfonyl-L-Arg methyl ester | - |
Hathewaya histolytica | |
0.22 | - |
benzoyl-Arg ethyl ester | - |
Hathewaya histolytica | |
0.25 | - |
Thr-Ala-Ala | - |
Hathewaya histolytica | |
1.3 | - |
N-benzoyl-L-Arg amide | - |
Hathewaya histolytica | |
2.5 | - |
p-Nitrophenyl p-guanidinobenzoate | - |
Hathewaya histolytica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | enhances esterase activity | Hathewaya histolytica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
- |
Hathewaya histolytica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hathewaya histolytica | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Hathewaya histolytica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Arg methyl ester + H2O | - |
Hathewaya histolytica | L-Arg + methanol | - |
? | |
additional information | the major activity is directed towards the carboxyl peptide linkage of Arg | Hathewaya histolytica | ? | - |
? | |
N-benzoyl-Arg amide + H2O | - |
Hathewaya histolytica | N-benzoyl-Arg + NH3 | - |
? | |
N-benzoyl-Arg benzyl ester + H2O | - |
Hathewaya histolytica | N-benzoyl-Arg + benzyl alcohol | - |
? | |
N-benzoyl-Arg ethyl ester + H2O | - |
Hathewaya histolytica | N-benzoyl-Arg + ethanol | - |
? | |
N-benzoyl-Arg isopropyl ester + H2O | - |
Hathewaya histolytica | N-benzoyl-Arg isopropanol | - |
? | |
N-benzoyl-Arg methyl ester + H2O | - |
Hathewaya histolytica | N-benzoyl-Arg + methanol | - |
? | |
Nalpha-benzoyl-L-Arg 2-naphthylamide + H2O | - |
Hathewaya histolytica | Nalpha-benzoyl-L-Arg + 2-naphthylamine | - |
? | |
p-nitrophenyl p-guanidinobenzoate + H2O | - |
Hathewaya histolytica | p-nitrophenol + p-guanidinobenzoate | - |
? | |
p-tosyl-Arg methyl ester + H2O | - |
Hathewaya histolytica | p-tosyl-Arg + methanol | - |
? | |
Thr-Ala-Ala + H2O | - |
Hathewaya histolytica | ? | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
Ca2+ depresses thermal inactivation | Hathewaya histolytica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
hydrolysis of L-Arg methyl ester, in phosphate buffer | Hathewaya histolytica |
7.4 | 7.8 | hydrolysis of alpha-benzoyl-Arg ethyl ester | Hathewaya histolytica |
7.4 | 7.8 | in phosphate or Tris-HCl buffer | Hathewaya histolytica |