Cloned (Comment) | Organism |
---|---|
overexpressed in Clostridium perfringens strain 13 | Clostridium perfringens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.852 | - |
benzoyl-L-Arg-4-nitroanilide | - |
Clostridium perfringens | |
25.4 | - |
Ac-L-Lys-4-nitroanilide | - |
Clostridium perfringens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
38000, 15000 /16000, SDS-PAGE, 38195, 14916 /15374, TOF mass spectrometry, generated on autocatalytic removal of the propeptide and linker undecapeptide from a single precursor peptide (light chains differ in the N-terminal cleavage site) | Clostridium perfringens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium perfringens | - |
- |
- |
Clostridium perfringens 13 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
cation-exchange chromatography (SP-Sepharose), ultrafiltration | Clostridium perfringens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.172 | - |
substrate benzoyl-L-Lys-4-nitroanilide | Clostridium perfringens |
4.28 | - |
substrate benzoyl-L-Arg-4-nitroanilide | Clostridium perfringens |
25.45 | - |
substrate Ac-L-Lys-4-nitroanilide | Clostridium perfringens |
123.5 | - |
substrate benzoyl-L-Arg-4-nitroanilide | Clostridium perfringens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Ac-L-Lys-4-nitroanilide + H2O | - |
Clostridium perfringens | N2-acetyl-L-Lys + 4-nitroaniline | - |
? | |
Ac-L-Lys-4-nitroanilide + H2O | - |
Clostridium perfringens 13 | N2-acetyl-L-Lys + 4-nitroaniline | - |
? | |
benzoyl-L-Arg-4-nitroanilide + H2O | - |
Clostridium perfringens | benzoyl-L-Arg + 4-nitroaniline | - |
? | |
benzoyl-L-Arg-4-nitroanilide + H2O | - |
Clostridium perfringens 13 | benzoyl-L-Arg + 4-nitroaniline | - |
? | |
benzoyl-Lys-4-nitroanilide + H2O | - |
Clostridium perfringens | benzoyl-Lys + 4-nitroaniline | - |
? | |
benzoyl-Lys-4-nitroanilide + H2O | - |
Clostridium perfringens 13 | benzoyl-Lys + 4-nitroaniline | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 38000, 15000 /16000, SDS-PAGE, 38195, 14916 /15374, TOF mass spectrometry, generated on autocatalytic removal of the propeptide and linker undecapeptide from a single precursor peptide (light chains differ in the N-terminal cleavage site) | Clostridium perfringens |
Synonyms | Comment | Organism |
---|---|---|
clostripain-like protease | - |
Clostridium perfringens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
22.53 | - |
Ac-L-Lys-4-nitroanilide | - |
Clostridium perfringens | |
109.3 | - |
benzoyl-L-Arg-4-nitroanilide | - |
Clostridium perfringens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
substrates: Bz-L-Arg-p-nitroanilide and Ac-L-Lys-4-nitroanilide | Clostridium perfringens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.887 | - |
Ac-L-Lys-4-nitroanilide | - |
Clostridium perfringens | |
128.3 | - |
benzoyl-L-Arg-4-nitroanilide | - |
Clostridium perfringens |