Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Staphylococcus aureus |
Protein Variants | Comment | Organism |
---|---|---|
I182A | the inhibitory effect of coptisine on mutants decreases in the order wild-type > R115A > I182A > N116A | Staphylococcus aureus |
N116A | the inhibitory effect of coptisine on mutants decreases in the order wild-type > R115A > I182A > N116A | Staphylococcus aureus |
R115A | the inhibitory effect of coptisine on mutants decreases in the order wild-type > R115A > I182A > N116A | Staphylococcus aureus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
coptisine | coptisine can bind to the active pocket of SrtB, residues Arg115, Asn116, and Ile182 play a vital role in the interaction of SrtB with coptisine. Coptisine can reduce the adhesion of Staphylococcus aureus to human lung epithelial cells | Staphylococcus aureus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | - |
- |
- |
Staphylococcus aureus ATCC 29213 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Dabcyl-QANPQTNEE-Edans + H2O | - |
Staphylococcus aureus | Dabcyl-QANPQT + NEE-Edans | - |
? | |
Dabcyl-QANPQTNEE-Edans + H2O | - |
Staphylococcus aureus ATCC 29213 | Dabcyl-QANPQT + NEE-Edans | - |
? |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
additional information | - |
IC50 value is 0.0087 mg/ml | Staphylococcus aureus | coptisine |