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BRENDA support

Literature summary for 3.4.22.70 extracted from

  • Levary, D.A.; Parthasarathy, R.; Boder, E.T.; Ackerman, M.E.
    Protein-protein fusion catalyzed by sortase A (2011), PLoS ONE, 6, e18342.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 cells Staphylococcus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
x * 27000, SDS-PAGE Staphylococcus sp.

Organism

Organism UniProt Comment Textmining
Staphylococcus sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Talon resin column chromatography Staphylococcus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
A33-LPETG-His6 + H2O A33 antigen extracellular domain bearing a His6 tag Staphylococcus sp. ?
-
?

Subunits

Subunits Comment Organism
? x * 27000, SDS-PAGE Staphylococcus sp.

General Information

General Information Comment Organism
physiological function sortase A covalently attaches proteins to the bacterial cell wall by cleaving between threonine and glycine at an LPXTG recognition motif Staphylococcus sp.