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Literature summary for 3.4.22.70 extracted from

  • Ito, T.; Sadamoto, R.; Naruchi, K.; Togame, H.; Takemoto, H.; Kondo, H.; Nishimura, S.
    Highly oriented recombinant glycosyltransferases: site-specific immobilization of unstable membrane proteins by using Staphylococcus aureus sortase A (2010), Biochemistry, 49, 2604-2614.
    View publication on PubMed
Search for more literature for 3.4.22.70

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Staphylococcus aureus

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
17800
-
 SDS-PAGE Staphylococcus aureus

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrapHP column chromatography, gel filtration Staphylococcus aureus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
AHLPKTGLR + 5-aminopentan-1-ol
-
 Staphylococcus aureus ?
-
 ?
AHLPKTGLR + 6-aminohexyl 4-O-beta-D-galactopyranosyl-beta-D-glucopyranoside
-
 Staphylococcus aureus ?
-
 ?
AHLPKTGLR + N-(2-(2-ethoxyethoxy)ethanamine)biotin amide
-
 Staphylococcus aureus ?
-
 ?
AHLPKTGLR + N-hexylbiotin amide
-
 Staphylococcus aureus ?
-
 ?
AHLPKTGLR-NH2 + triglycine
-
 Staphylococcus aureus ?
-
 ?
additional information the enzyme cleaves the LPXTG sequence at the amide bond between the threonine and the glycine to form an acyl-enzyme complex. Nucleophilic attack by the amino group of the tri-glycine on the intermediate results in the formation of an LPXT-GGG bond and the liberation of the free enzyme Staphylococcus aureus ?
-
 ?
recombinant Helicobacter pylori R1,3-fucosyltransferase + triglycine
-
 Staphylococcus aureus ?
-
 ?
recombinant human beta1,4-galactosyltransferase + N-(2-(2-ethoxyethoxy)ethanamine)biotin amide
-
 Staphylococcus aureus ?
-
 ?
recombinant human beta1,4-galactosyltransferase + N-hexylbiotin amide
-
 Staphylococcus aureus ?
-
 ?

Synonyms

Synonyms Comment Organism
SrtA
-
 Staphylococcus aureus