Application | Comment | Organism |
---|---|---|
molecular biology | method for immobilizing ligand proteins onto Biacore sensor chips using the transpeptidase activity of Staphylococcus aureus sortase A. This method provides a robust and gentle approach for the site-directed, covalent coupling of proteins to biosensor chips. The high specificity of the sortase allows immobilization of proteins from less than pure protein samples allowing short cuts in protein purification protocols | Staphylococcus aureus |
Cloned (Comment) | Organism |
---|---|
recombinant mature sortase A (without the N-terminal signal peptide) is produced as GST fusion in Escherichia coli | Staphylococcus aureus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant sortase A-GST fusion protein | Staphylococcus aureus |
Synonyms | Comment | Organism |
---|---|---|
SrtA | - |
Staphylococcus aureus |