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Literature summary for 3.4.22.70 extracted from

  • Kruger, R.G.; Otvos, B.; Frankel, B.A.; Bentley, M.; Dostal, P.; McCafferty, D.G.
    Analysis of the substrate specificity of the Staphylococcus aureus sortase transpeptidase SrtA (2004), Biochemistry, 43, 1541-1551.
    View publication on PubMed
Search for more literature for 3.4.22.70

Application

Application Comment Organism
medicine SrtA activity is a prime target for inhibition of Staphylococcus aureus colonization Staphylococcus aureus

Cloned(Commentary)

Cloned (Comment) Organism
expression of SrtADELTAN24 in a His6-tagged form Staphylococcus aureus

Inhibitors

Inhibitors Comment Organism Structure
additional information SrtA activity is a prime target for inhibition of Staphylococcus aureus colonization Staphylococcus aureus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.137
-
 Gly
-
 Staphylococcus aureus
5.5
-
 Abz-LPETG-Dap(Dnp)-NH2
-
 Staphylococcus aureus
5.6
-
 Dnp-AQALPETGEE-NH2
-
 Staphylococcus aureus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Staphylococcus aureus primary role of the SrtA isoform in Staphylococcus aureus adhesion and host colonization ?
-
 ?

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant SrtADELTAN24 in a His6-tagged form Staphylococcus aureus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0255
-
-
 Staphylococcus aureus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Abz-LPETG-Dap(Dnp)-NH2 + Gly5
-
 Staphylococcus aureus Abz-LPETGGGGG-OH + Gly-Dap(Dnp)-NH2
-
 ?
Dnp-AQALPETGEE-NH2 + Gly5
-
 Staphylococcus aureus ?
-
 ?
additional information primary role of the SrtA isoform in Staphylococcus aureus adhesion and host colonization Staphylococcus aureus ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.013
-
 Dnp-AQALPETGEE-NH2
-
 Staphylococcus aureus
0.27
-
 Abz-LPETG-Dap(Dnp)-NH2
-
 Staphylococcus aureus