KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0097 | - |
N-carbobenzoxy-Lys p-nitrophenyl ester | asclepain A5 | Asclepias syriaca | |
0.0105 | - |
N-carbobenzoxy-Lys p-nitrophenyl ester | asclepain A2 | Asclepias syriaca | |
0.0114 | - |
N-carbobenzoxy-Lys p-nitrophenyl ester | asclepain A1 | Asclepias syriaca | |
0.0122 | - |
N-carbobenzoxy-Lys p-nitrophenyl ester | asclepain A4 | Asclepias syriaca | |
0.0126 | - |
N-carbobenzoxy-Lys p-nitrophenyl ester | asclepain B3 | Asclepias syriaca | |
0.0142 | - |
N-carbobenzoxy-Lys p-nitrophenyl ester | asclepain A3 | Asclepias syriaca | |
0.0248 | - |
N-carbobenzoxy-Lys p-nitrophenyl ester | asclepain B1 | Asclepias syriaca | |
0.027 | - |
N-carbobenzoxy-Lys p-nitrophenyl ester | asclepain B4 | Asclepias syriaca | |
0.0272 | - |
N-carbobenzoxy-Lys p-nitrophenyl ester | asclepain B5 | Asclepias syriaca | |
0.0389 | - |
N-carbobenzoxy-Lys p-nitrophenyl ester | asclepain B2 | Asclepias syriaca |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
21000 | - |
x * 21000, asclepain A, SDS-PAGE | Asclepias syriaca |
23000 | - |
x * 23000, asclepain B, SDS-PAGE | Asclepias syriaca |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Asclepias syriaca | - |
asclepain A1, A2, A3, A4, A5, and asclepain B1, B2, B3, B4 and B5 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
latex | - |
Asclepias syriaca | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-carbobenzoxy-Lys p-nitrophenyl ester + H2O | - |
Asclepias syriaca | N-carbobenzoxy-Lys + 4-nitrophenol | - |
? | |
Phe-Val-Asn-Ala-His-Leu-CysA-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-CysA-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala + H2O | - |
Asclepias syriaca | Phe-Val-Asn + Ser-His-Leu-Val-Glu-Ala-Leu + Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-CysA-Gly-Glu + Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala + Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala + Gly-Phe-Phe-Tyr-Thr | 14% Phe-Val-Asn + 22% Ser-His-Leu-Val-Glu-Ala-Leu + 15% Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-CysA-Gly-Glu + 10% Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala + 10% Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala + 13% Gly-Phe-Phe-Tyr-Thr | ? |
Subunits | Comment | Organism |
---|---|---|
? | x * 21000, asclepain A, SDS-PAGE | Asclepias syriaca |
? | x * 23000, asclepain B, SDS-PAGE | Asclepias syriaca |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
22.3 | - |
N-carbobenzoxy-Lys p-nitrophenyl ester | asclepain A5 | Asclepias syriaca | |
25.8 | - |
N-carbobenzoxy-Lys p-nitrophenyl ester | asclepain B3 | Asclepias syriaca | |
33.8 | - |
N-carbobenzoxy-Lys p-nitrophenyl ester | asclepain B4 | Asclepias syriaca | |
34.6 | - |
N-carbobenzoxy-Lys p-nitrophenyl ester | asclepain B1 | Asclepias syriaca | |
42.9 | - |
N-carbobenzoxy-Lys p-nitrophenyl ester | asclepain A3 | Asclepias syriaca | |
46.2 | - |
N-carbobenzoxy-Lys p-nitrophenyl ester | asclepain A2 | Asclepias syriaca | |
47.6 | - |
N-carbobenzoxy-Lys p-nitrophenyl ester | asclepain B5 | Asclepias syriaca | |
49.2 | - |
N-carbobenzoxy-Lys p-nitrophenyl ester | asclepain B2 | Asclepias syriaca | |
54.7 | - |
N-carbobenzoxy-Lys p-nitrophenyl ester | asclepain A1 | Asclepias syriaca | |
59.6 | - |
N-carbobenzoxy-Lys p-nitrophenyl ester | asclepain A4 | Asclepias syriaca |