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Literature summary for 3.4.22.7 extracted from

  • Liggieri, C.; Arribere, M.C.; Trejo, S.A.; Canals, F.; Aviles, F.X.; Priolo, N.S.
    Purification and biochemical characterization of asclepain c I from the latex of Asclepias curassavica L (2004), Protein J., 23, 403-411.
    View publication on PubMed
Search for more literature for 3.4.22.7

Inhibitors

Inhibitors Comment Organism Structure
E-64
-
 Asclepias curassavica
NaCl 1 M, 70% loss of activity Asclepias curassavica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.73
-
 L-pyroglutamyl-L-phenylalanyl-L-leucine-4-nitroanilide pH 8.5, 42°C, Lineweaver-Burk equation Asclepias curassavica
0.82
-
 L-pyroglutamyl-L-phenylalanyl-L-leucine-4-nitroanilide pH 8.5, 42°C, Eddie-Hofstee equation Asclepias curassavica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23200
-
 x * 23200, mass spectrometry Asclepias curassavica

Organism

Organism UniProt Comment Textmining
Asclepias curassavica
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
latex
-
 Asclepias curassavica
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
10880
-
 pH 8.5, 42°C Asclepias curassavica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
azocasein + H2O
-
 Asclepias curassavica ?
-
 ?
L-pyroglutamyl-L-phenylalanyl-L-leucine-4-nitroanilide + H2O
-
 Asclepias curassavica ?
-
 ?
N-carbobenzoxy-L-Ala 4-nitrophenyl ester + H2O 47% of the activity with N-carbobenzoxy-L-Gln 4-nitrophenyl ester Asclepias curassavica N-carbobenzoxy-L-Ala + 4-nitrophenol
-
 ?
N-carbobenzoxy-L-Gln 4-nitrophenyl ester + H2O
-
 Asclepias curassavica N-carbobenzoxy-L-Gln + 4-nitrophenol
-
 ?
N-carbobenzoxy-L-Ile 4-nitrophenyl ester + H2O 59% of the activity with N-carbobenzoxy-L-Gln 4-nitrophenyl ester Asclepias curassavica N-carbobenzoxy-L-Ile + 4-nitrophenol
-
 ?
N-carbobenzoxy-L-Lys 4-nitrophenyl ester + H2O 78% of the activity with N-carbobenzoxy-L-Gln 4-nitrophenyl ester Asclepias curassavica N-carbobenzoxy-L-Lys + 4-nitrophenol
-
 ?
N-carbobenzoxy-L-Tyr 4-nitrophenyl ester + H2O 51% of the activity with N-carbobenzoxy-L-Gln 4-nitrophenyl ester Asclepias curassavica N-carbobenzoxy-L-Tyr + 4-nitrophenol
-
 ?

Subunits

Subunits Comment Organism
? x * 23200, mass spectrometry Asclepias curassavica

Synonyms

Synonyms Comment Organism
asclepain cI
-
 Asclepias curassavica

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 2 h at pH 8.5, stable, 4 h, 30% loss of activity Asclepias curassavica
40
-
 2 h, stable Asclepias curassavica
50
-
 2 h, stable Asclepias curassavica
60
-
 2 h, stable Asclepias curassavica
70
-
 40 min, complete inactivation Asclepias curassavica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Asclepias curassavica

pH Range

pH Minimum pH Maximum Comment Organism
7.5 9
-
 Asclepias curassavica

pH Stability

pH Stability pH Stability Maximum Comment Organism
8.5
-
 2 h at 37°C, stable, 4 h, 30% loss of activity Asclepias curassavica

pI Value

Organism Comment pI Value Maximum pI Value
Asclepias curassavica higher than, isoelectric focusing
-
 9.3