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Literature summary for 3.4.22.69 extracted from

  • Li, C.; Teng, X.; Qi, Y.; Tang, B.; Shi, H.; Ma, X.; Lai, L.
    Conformational flexibility of a short loop near the active site of the SARS-3CLpro is essential to maintain catalytic activity (2016), Sci. Rep., 6, 20918 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.22.69

Cloned(Commentary)

Cloned (Comment) Organism
-
 Severe acute respiratory syndrome-related coronavirus

Protein Variants

Protein Variants Comment Organism
G11A/L141T/R298A/Q299A mutant is enzymatically active as a monomer, about 2% of wild-type activity Severe acute respiratory syndrome-related coronavirus
G11A/R298A/Q299A inactive Severe acute respiratory syndrome-related coronavirus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.68
-
 Thr-Ser-Ala-Val-Leu-Gln-4-nitroanilide mutant G11A/L141T/R298A/Q299A, pH 7.3, 37°C Severe acute respiratory syndrome-related coronavirus
0.98
-
 Thr-Ser-Ala-Val-Leu-Gln-4-nitroanilide wild-type, pH 7.3, 37°C Severe acute respiratory syndrome-related coronavirus

Organism

Organism UniProt Comment Textmining
Severe acute respiratory syndrome-related coronavirus
-
 human, i.e. SARS-CoV
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Thr-Ser-Ala-Val-Leu-Gln-4-nitroanilide + H2O
-
 Severe acute respiratory syndrome-related coronavirus Thr-Ser-Ala-Val-Leu-Gln + 4-nitroaniline
-
 ?

Subunits

Subunits Comment Organism
More the Ser139-Leu141 loop forms a short 310-helix that disrupts the catalytic machinery in the inactive monomer structure. Disruption of this helical conformation by mutating L141 to T in the stable inactive monomer G11A/R298A/Q299A leads to tetra-mutant G11A/L141T/R298A/Q299A which is enzymatically active as a monomer. The L141T mutation disrupts the 310-helix and helps to stabilize the active conformation. The coil-310-helix conformational transition of the Ser139-Leu141 loop serves as an enzyme activity switch Severe acute respiratory syndrome-related coronavirus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0015
-
 Thr-Ser-Ala-Val-Leu-Gln-4-nitroanilide mutant G11A/L141T/R298A/Q299A, pH 7.3, 37°C Severe acute respiratory syndrome-related coronavirus
0.11
-
 Thr-Ser-Ala-Val-Leu-Gln-4-nitroanilide wild-type, pH 7.3, 37°C Severe acute respiratory syndrome-related coronavirus