Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | enzyme stimulation occurs in two steps, with approximately 8fold stimulation by N-terminal cleavage, approximately 4fold stimulation by C-terminal cleavage, and 23fold stimulation by the cleavage of both termini, compared to the pro-form with both the N- and C-terminal pro-sequences | Severe acute respiratory syndrome-related coronavirus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | development of a SARS 3CL protease autoprocessing system by use of the Escherichia coli cell-free protein synthesis system, with the N- and C-terminal 10 amino acid pro-sequences accompanied by an S-tag and a His-tag, respectively | Severe acute respiratory syndrome-related coronavirus |
Q1N | site-directed mutagenesis, the mutation results in loss of cleavage at this site | Severe acute respiratory syndrome-related coronavirus |
Q306N | site-directed mutagenesis, the mutation results in loss of cleavage at this site | Severe acute respiratory syndrome-related coronavirus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
2 * 34000, mature, catalytically active enzyme, SDS-PAGE, SARS-CoV 3CLpro exists as a homodimer, in which each protomer has an active site | Severe acute respiratory syndrome-related coronavirus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Severe acute respiratory syndrome-related coronavirus | - |
SARS CoV | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | the enzyme self-activates via autoprocessing, analysis using enzyme pro-forms with the N-terminal pro-sequence, the C-terminal pro-sequence or both pro-sequences as catalyst toward the pro-form with both N- and C-terminal pro-sequences, mechanism, overview. The stimulation occurs in two steps, with approximately 8fold stimulation by N-terminal cleavage, approximately 4fold stimulation by C-terminal cleavage, and 23fold stimulation by the cleavage of both termini, compared to the pro-form with both the N- and C-terminal pro-sequences. Such cleavage mainly occurs in a trans manner, i.e. the pro-form dimer cleaves the monomeric form. C-terminal cleavage is due to removal of its trans (inter-dimer) inhibitory effect | Severe acute respiratory syndrome-related coronavirus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme performs autoprocessing, overview. As 3CLpro requires a glutamine residue at the P1 position of the substrate | Severe acute respiratory syndrome-related coronavirus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 34000, mature, catalytically active enzyme, SDS-PAGE, SARS-CoV 3CLpro exists as a homodimer, in which each protomer has an active site | Severe acute respiratory syndrome-related coronavirus |
Synonyms | Comment | Organism |
---|---|---|
3C-like protease | - |
Severe acute respiratory syndrome-related coronavirus |
3cLpro | - |
Severe acute respiratory syndrome-related coronavirus |
main protease | - |
Severe acute respiratory syndrome-related coronavirus |
Mpro | - |
Severe acute respiratory syndrome-related coronavirus |
SARS 3CL protease | - |
Severe acute respiratory syndrome-related coronavirus |
SARS-CoV 3CLpro | - |
Severe acute respiratory syndrome-related coronavirus |
severe acute respiratory syndrome coronavirus 3C-like protease | - |
Severe acute respiratory syndrome-related coronavirus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
autocatalytic assay at | Severe acute respiratory syndrome-related coronavirus |
General Information | Comment | Organism |
---|---|---|
additional information | autoprocessing mechanism of the enzyme | Severe acute respiratory syndrome-related coronavirus |