Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.22.69 extracted from

  • Li, C.; Qi, Y.; Teng, X.; Yang, Z.; Wei, P.; Zhang, C.; Tan, L.; Zhou, L.; Liu, Y.; Lai, L.
    Maturation mechanism of severe acute respiratory syndrome (SARS) coronavirus 3C-like proteinase (2010), J. Biol. Chem., 285, 28134-28140.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.22.69

Cloned(Commentary)

Cloned (Comment) Organism
His-tagged artificial polyprotein (cyan fluorescent protein-SARS-CoV 3CLpro-yellow fluorescent protein) expressed in Escherichia coli Severe acute respiratory syndrome-related coronavirus

Inhibitors

Inhibitors Comment Organism Structure
1-(2-naphthylmethyl)isatin-5-carboxamide
-
 Severe acute respiratory syndrome-related coronavirus

Organism

Organism UniProt Comment Textmining
Severe acute respiratory syndrome-related coronavirus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Thr-Ser-Ala-Val-Leu-Gln-p-nitroanilide + H2O
-
 Severe acute respiratory syndrome-related coronavirus Thr-Ser-Ala-Val-Leu-Gln + p-nitroaniline
-
 ?

Subunits

Subunits Comment Organism
homodimer tendency of substrate induced dimer formation, gel filtration, analytic ultracentrifugation Severe acute respiratory syndrome-related coronavirus

Synonyms

Synonyms Comment Organism
SARS coronavirus 3C-like proteinase
-
 Severe acute respiratory syndrome-related coronavirus
SARS-3CLpro
-
 Severe acute respiratory syndrome-related coronavirus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00037
-
-
 Severe acute respiratory syndrome-related coronavirus 1-(2-naphthylmethyl)isatin-5-carboxamide

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.06
-
 Thr-Ser-Ala-Val-Leu-Gln-p-nitroanilide His-tagged artificial polyprotein (cyan fluorescent protein-SARS-CoV 3CLpro-yellow fluorescent protein) Severe acute respiratory syndrome-related coronavirus
0.11
-
 Thr-Ser-Ala-Val-Leu-Gln-p-nitroanilide His-tagged artificial polyprotein (SARS-CoV 3CLpro-yellow fluorescent protein) Severe acute respiratory syndrome-related coronavirus
0.53
-
 Thr-Ser-Ala-Val-Leu-Gln-p-nitroanilide SARS-CoV 3CLpro Severe acute respiratory syndrome-related coronavirus