Literature summary for 3.4.22.69 extracted from

Graziano, V.; McGrath, W.J.; DeGruccio, A.M.; Dunn, J.J.; Mangel, W.F.
Enzymatic activity of the SARS coronavirus main proteinase dimer (2006), FEBS Lett., 580, 2577-2583.

Search for more literature for 3.4.22.69

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in E. coli BL21	Severe acute respiratory syndrome-related coronavirus

Inhibitors

Inhibitors	Comment	Organism	Structure
DTT	80% of enzyme activity inhibited in the presence of 2.5 mM DTT	Severe acute respiratory syndrome-related coronavirus
NaCl	80% of enzyme activity inhibited in the presence of 100 mM NaCl	Severe acute respiratory syndrome-related coronavirus
NP-40	80% of enzyme activity inhibited in the presence of 0.1% NP-40	Severe acute respiratory syndrome-related coronavirus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.306	-	(Ala-Arg-Leu-Gln-NH)2-rhodamine	rate of hydrolysis measured by change in absorbance at 496 nm	Severe acute respiratory syndrome-related coronavirus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
33760	-	determined by MALDI	Severe acute respiratory syndrome-related coronavirus

Organism

Organism	UniProt	Comment	Textmining
Severe acute respiratory syndrome-related coronavirus	-	virus infected Vero cells	-

Purification (Commentary)

Purification (Comment)	Organism
strong cation column chromatography connected in series to a strong anion column chromatography	Severe acute respiratory syndrome-related coronavirus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(Ala-Arg-Leu-Gln-NH)2-rhodamine	-	Severe acute respiratory syndrome-related coronavirus	rhodamine 110 + (Ala-Arg-Leu-Gln-NH)-rhodamine	-	?

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
42	-	-	Severe acute respiratory syndrome-related coronavirus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.0153	-	(Ala-Arg-Leu-Gln-NH)2-rhodamine	rate of hydrolysis measured by change in absorbance at 496 nm	Severe acute respiratory syndrome-related coronavirus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	-	Severe acute respiratory syndrome-related coronavirus

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Release 2023.1 (January 2023)