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Literature summary for 3.4.22.67 extracted from
- Zingipain, a ginger protease with acetylcholinesterase inhibitory activity (2013), Appl. Biochem. Biotechnol., 170, 934-950.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | about 60% inhibition at 1-10 mM | Zingiber officinale |  |
| Fe2+ | activates at 1 mM, inhibits at 10 mM | Zingiber officinale | |
| Hg2+ | activates at 1 mM, complete inhibition at 5-10 mM | Zingiber officinale | |
| Mg2+ | activates at 1 mM, inhibits at 10 mM | Zingiber officinale | |
| Zn2+ | activates at 1 mM, inhibits at 10 mM | Zingiber officinale | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | activates at 1-10 mM | Zingiber officinale | |
| Fe2+ | activates at 1 mM, inhibits at 10 mM | Zingiber officinale | |
| Hg2+ | activates at 1 mM, complete inhibition at 5-10 mM | Zingiber officinale | |
| Mg2+ | activates at 1 mM, inhibits at 10 mM | Zingiber officinale | |
| Mn2+ | activates at 1-10 mM | Zingiber officinale | |
| Zn2+ | activates at 1 mM, inhibits at 10 mM | Zingiber officinale | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33500 | - |
native PAGE | Zingiber officinale |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Zingiber officinale | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme from rhizomes by saturation ammonium sulfate fractionation and anion exchange chromatography to homogeneity | Zingiber officinale |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| rhizome | - |
Zingiber officinale | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20-60, purified native enzyme, at least 120 min, stable | Zingiber officinale |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| azocasein + H2O | - |
Zingiber officinale | ? | - |
? | |
| Gelatin + H2O | - |
Zingiber officinale | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 33500, SDS-PAGE | Zingiber officinale |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ginger protease | - |
Zingiber officinale |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Zingiber officinale |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| -20 | 60 | purified native enzyme, at least 120 min, stable | Zingiber officinale |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
assay at | Zingiber officinale |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 2 | 12 | purified native enzyme, at least 120 min, stable | Zingiber officinale |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme has sequence similarity with zingipain-1 from Zingiber officinalis | Zingiber officinale |
| additional information | the enzyme has cysteine protease activity, but also a significant acetylcholinesterase inhibitor activity exhibiting noncompetitive inhibition of acetylcholinesterase for the hydrolysis of acetylthiocholine iodide with a Ki value of 9.31 mg/ml., overview | Zingiber officinale |
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Release 2023.1 (January 2023)
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