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Literature summary for 3.4.22.66 extracted from

  • Zeitler, C.E.; Estes, M.K.; Venkataram Prasad, B.V.
    X-ray crystallographic structure of the Norwalk virus protease at 1.5-A resolution (2006), J. Virol., 80, 5050-5058.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.22.66

Activating Compound

Activating Compound Comment Organism Structure
Glu 54 active role in proteolysis Norwalk virus

Application

Application Comment Organism
medicine Norwalk virus is the major cause of acute, epidemic, viral gastroenteritis Norwalk virus

Cloned(Commentary)

Cloned (Comment) Organism
cloned into pET41 Ek/LIC and pET46 Ek/LIC and expressed in Escherichia coli BL21 (DE3) Norwalk virus

Crystallization (Commentary)

Crystallization (Comment) Organism
by hanging drop vapor diffusion or sitting drop vapor diffusion at room temperature, to 1.5 and 2.2 A resolution Norwalk virus

Protein Variants

Protein Variants Comment Organism
E54A Glu at the active centre Norwalk virus

Inhibitors

Inhibitors Comment Organism Structure
4-(2-aminoethyl)-benzene-sulfonyl fluoride irreversible inhibition, soluble stable nontoxic alternative to phenylmethylsulfonyl fluoride Norwalk virus
phenylmethylsulfonyl fluoride
-
 Norwalk virus

Organism

Organism UniProt Comment Textmining
Norwalk virus Q83883
-
-

Purification (Commentary)

Purification (Comment) Organism
by nickel affinity chromatography or size exclusion chromatography Norwalk virus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ORF1 polyprotein + H2O
-
 Norwalk virus six nonstructural proteins for replication
-
 ?

Subunits

Subunits Comment Organism
dimer size exclusion chromatography results Norwalk virus

Synonyms

Synonyms Comment Organism
NV protease
-
 Norwalk virus
NVPro
-
 Norwalk virus
viral cysteine protease
-
 Norwalk virus