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Literature summary for 3.4.22.65 extracted from
- Production of a chimeric allergen derived from the major allergen group 1 of house dust mite species in Nicotiana benthamiana (2013), Hum. Immunol., 74, 531-537.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant expression of C-terminally His6-tagged chimeric allergen R8, derived from the major allergen group 1 of house dust mites species, Dermatophagoides farinae and Dermatophagoides pteronyssinus, in leaves of Nicotiana benthamiana via transfection with Agrobacterium tumefaciens strain GV3101 | Dermatophagoides pteronyssinus |
| recombinant expression of C-terminally His6-tagged wild-type enzyme and chimeric allergen R8, derived from the major allergen group 1 of house dust mites species, Dermatophagoides farinae and Dermatophagoides pteronyssinus, in leaves of Nicotiana benthamiana via transfection with Agrobacterium tumefaciens strain GV3101 | Dermatophagoides farinae |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of a chimeric allergen R8, derived from the major allergen group 1 of house dust mites species, Dermatophagoides farinae and Dermatophagoides pteronyssinus | Dermatophagoides pteronyssinus |
| additional information | construction of a chimeric allergen R8, derived from the major allergen group 1 of house dust mites species, Dermatophagoides farinae and Dermatophagoides pteronyssinus | Dermatophagoides farinae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Dermatophagoides farinae | Q58A71 | preproenzyme | - |
| Dermatophagoides pteronyssinus | P08176 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His6-tagged chimeric allergen R8 from Nicotiana benthamiana leaves by affinity chromatography to homogeneity under denaturing conditions | Dermatophagoides pteronyssinus |
| recombinant His6-tagged wild-type enzyme and chimeric allergen R8 from Nicotiana benthamiana leaves by affinity chromatography to homogeneity under denaturing conditions | Dermatophagoides farinae |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| recombinant His6-tagged chimeric allergen R8 from Nicotiana benthamiana leaves after purification by affinity chromatography under denaturing conditions, refolding by dialysis against decreasing concentrations of urea | Dermatophagoides pteronyssinus |
| recombinant His6-tagged wild-type enzyme and chimeric allergen R8 from Nicotiana benthamiana leaves after purification by affinity chromatography under denaturing conditions, refolding by dialysis against decreasing concentrations of urea | Dermatophagoides farinae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ProDer f 1 | - |
Dermatophagoides farinae |
| ProDer p 1 | - |
Dermatophagoides pteronyssinus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | the enzyme is a group 1 mite allergen | Dermatophagoides pteronyssinus |
| additional information | the enzyme is a group 1 mite allergen | Dermatophagoides farinae |
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Release 2023.1 (January 2023)
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