Application | Comment | Organism |
---|---|---|
medicine | chimeras assembling Der p 1 and Der p 2 allergens are potential candidate vaccines against house dust mite allergy. But significant solubility and stability issues can limit the application of such chimeras for immunotherapy or diagnostic | Dermatophagoides pteronyssinus |
Cloned (Comment) | Organism |
---|---|
expression of His-tagged fusion proteins encompassing Der p 2 with either mature or proDer p 1 in Escherichia coli strain BL21 (DE3) and Origami2 (DE3), or in Pichia pastoris strain GS115 or X33. Four fusion proteins are expressed in Escherichia coli as inclusion bodies, whereas only chimeras comprising proDer p 1 are obtained in yeast | Dermatophagoides pteronyssinus |
Protein Variants | Comment | Organism |
---|---|---|
C132V | site-directed mutagenesis, Der p 1 catalytic site mutant | Dermatophagoides pteronyssinus |
additional information | development of chimeras assembling Der p 1 and Der p 2 allergens. Mutations in the Der p 1 catalytic site are also engineered | Dermatophagoides pteronyssinus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dermatophagoides pteronyssinus | P49273 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged fusion proteins by nickel affinity chromatography using a His-Trap column under denaturing conditions with 20 m M sodium phosphate, pH 7.0, 0.5 M NaCl, 0.05% Tween 20, 8 M urea, 1 mM DTT, 0.03 M imidazole as binding buffer, and 20 mM sodium phosphate, pH 7.8, 0.05% Tween 20, 8 M urea, 1 mM DTT, 1 M imidazole as elution buffer | Dermatophagoides pteronyssinus |
Renatured (Comment) | Organism |
---|---|
recombinant His-tagged fusion proteins encompassing Der p 2 with either mature or proDer p 1 from Escherichia coli inclusion bodies with purification via denaturating nickel affinity chromatography | Dermatophagoides pteronyssinus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Z-Phe-Arg-4-methylcoumarin 7-amide + H2O | - |
Dermatophagoides pteronyssinus | Z-Phe-Arg + 7-amino-4-methylcoumarin | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Dermatophagoides pteronyssinus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Dermatophagoides pteronyssinus |
General Information | Comment | Organism |
---|---|---|
physiological function | the isozymes have allergenic potential in humans | Dermatophagoides pteronyssinus |