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Literature summary for 3.4.22.65 extracted from

  • Takai, T.; Mineki, R.; Nakazawa, T.; Takaoka, M.; Yasueda, H.; Murayama, K.; Okumura, K.; Ogawa, H.
    Maturation of the activities of recombinant mite allergens Der p 1 and Der f 1, and its implication in the blockade of proteolytic activity (2002), FEBS Lett., 531, 265-272.
    View publication on PubMed
Search for more literature for 3.4.22.65

Cloned(Commentary)

Cloned (Comment) Organism
prepro-Der f1 Dermatophagoides farinae
wild-type and mutant enzyme N52Q, expression in Pichia pastoris Dermatophagoides pteronyssinus

Protein Variants

Protein Variants Comment Organism
N52Q mutant enzyme has no yeast-derived N-glycosylation Dermatophagoides pteronyssinus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24000
-
 x * 24000, mature mutant enzyme N52Q, SDS-PAGE under non-reducing conditions Dermatophagoides pteronyssinus

Organism

Organism UniProt Comment Textmining
Dermatophagoides farinae
-
-
-
Dermatophagoides pteronyssinus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Dermatophagoides farinae
proteolytic modification the proforms of Der p 1 and Der f1 differ in the N-terminal sequences Dermatophagoides farinae
proteolytic modification contribution of the N-terminal region of the Der p 1 prosequence including the N-glycosylation site, Asn(-65), on effective inhibition of proteolytic activity in pro-Der p 1 Dermatophagoides pteronyssinus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
butyloxycarbonyl-Gln-Ala-Arg-4-methylcoumaryl-7-amide + H2O
-
 Dermatophagoides pteronyssinus butyloxycarbonyl-Gln-Ala-Arg + 7-amino-4-methylcoumarin
-
 ?

Subunits

Subunits Comment Organism
? x * 34000-45000, mature enzyme, SDS-PAGE Dermatophagoides farinae
? x * 24000, mature mutant enzyme N52Q, SDS-PAGE under non-reducing conditions Dermatophagoides pteronyssinus
? x * 40000-50000, mature mutant enzyme N52Q, SDS-PAGE under non-reducing conditions Dermatophagoides pteronyssinus
? x * 35000-45000, mature wild-type enzyme, SDS-PAGE under non-reducing conditions Dermatophagoides pteronyssinus
? x * 35000-45000, mature wild-type enzyme under reducing conditions Dermatophagoides pteronyssinus