Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.22.62 extracted from

  • de Witte, M.A.; Jorritsma, A.; Swart, E.; Straathof, K.C.; de Punder, K.; Haanen, J.B.; Rooney, C.M.; Schumacher, T.N.
    An inducible caspase 9 safety switch can halt cell therapy-induced autoimmune disease (2008), J. Immunol., 180, 6365-6373.
    View publication on PubMed
Search for more literature for 3.4.22.62

Application

Application Comment Organism
medicine effectiveness and specificity of an inducible caspase 9-based safety switch system, to halt an ongoing immune attack, reduction of the risk of severe graft-vs-host disease, murine model for cell therapy-induced type I diabetes Mus musculus

Cloned(Commentary)

Cloned (Comment) Organism
B6 splenocytes modified by retroviral transduction, truncated caspase-9 molecule genetically coupled to a modified human FK506-binding protein (FKBPF36V) by generating the iCasp9M-construct, iCasp9M construct (safety switch) and enhanced GFP used for transformation Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus Q9R0T0 murine model for cell therapy-induced type I diabetes
-

Source Tissue

Source Tissue Comment Organism Textmining
splenocyte B6 mice, modified by retroviral transduction Mus musculus
-
T-lymphocyte retroviral transduction of Mus musculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 value of an inducible caspase-9-based safety switch to halt an ongoing immune attack, retroviral transduction of T cells, induction and analysis of apoptosis, depletion of transduced T cells determined by flow cytometric analysis of GFP expression, in vivo assessment of the inducible caspase-9 suicide switch, immunohistochemistry, evaluation of this conditional safety switch in clinical trials of adoptive cell therapy Mus musculus